Instillation of allogeneic lung antigen-presenting cells deficient in expression of major histocompatibility complex class I or II antigens have differential effects on local cellular and humoral immunity and on pathology in recipient murine lungs

K. M. Heidler, K. Baker, K. Woods, C. Schnizlein-Bick, Oscar Cummings, R. Sidner, B. Foresman, D. S. Wilkes

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Recognition of allogeneic major histocompatibility complex (MHC) molecules expressed on donor lung antigen-presenting cells (APCs) by host T lymphocytes is believed to stimulate lung allograft rejection. However, the specific roles of donor MHC molecules in the rejection response is unknown. We report a murine model in which instilling allogeneic lung APCs into recipient lungs induces pathology analogous to acute rejection, and the production of interferon (IFN)-γ, immunoglobulin (Ig) G2a, and alloantibodies in recipient lungs. Using allogeneic lung APCs (C57BL/6, I-ab, H-2b) deficient in MHC class I, II, or both for instillation into lungs of BALB/c mice (I-a(d), H-2(d)), the purpose of the current study was to determine the specific roles of donor MHC molecules in stimulating local alloimmune responses. The data show that MHC class I or II on donor APCs induced IFN-γ and IgG2a synthesis locally, though less than that induced by wild-type cells. Both MHC class I and II were required to induce alloantibody production. Instillation of wild-type or class I- or class II-deficient APCs induced comparable pathologic lesions in recipient lungs, and more severe than that induced by MHC-deficient cells. These data show that donor MHC class I and II molecules have differential effects in the stimulation of local alloimmune responses.

Original languageEnglish
Pages (from-to)499-505
Number of pages7
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume23
Issue number4
StatePublished - 2000

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Histocompatibility Antigens Class I
Histocompatibility Antigens Class II
Pathology
Antigen-Presenting Cells
Humoral Immunity
Major Histocompatibility Complex
Cellular Immunity
Lung
Isoantibodies
Molecules
Interferons
T-cells
Allografts
Immunoglobulins
T-Lymphocytes

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Pulmonary and Respiratory Medicine

Cite this

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title = "Instillation of allogeneic lung antigen-presenting cells deficient in expression of major histocompatibility complex class I or II antigens have differential effects on local cellular and humoral immunity and on pathology in recipient murine lungs",
abstract = "Recognition of allogeneic major histocompatibility complex (MHC) molecules expressed on donor lung antigen-presenting cells (APCs) by host T lymphocytes is believed to stimulate lung allograft rejection. However, the specific roles of donor MHC molecules in the rejection response is unknown. We report a murine model in which instilling allogeneic lung APCs into recipient lungs induces pathology analogous to acute rejection, and the production of interferon (IFN)-γ, immunoglobulin (Ig) G2a, and alloantibodies in recipient lungs. Using allogeneic lung APCs (C57BL/6, I-ab, H-2b) deficient in MHC class I, II, or both for instillation into lungs of BALB/c mice (I-a(d), H-2(d)), the purpose of the current study was to determine the specific roles of donor MHC molecules in stimulating local alloimmune responses. The data show that MHC class I or II on donor APCs induced IFN-γ and IgG2a synthesis locally, though less than that induced by wild-type cells. Both MHC class I and II were required to induce alloantibody production. Instillation of wild-type or class I- or class II-deficient APCs induced comparable pathologic lesions in recipient lungs, and more severe than that induced by MHC-deficient cells. These data show that donor MHC class I and II molecules have differential effects in the stimulation of local alloimmune responses.",
author = "Heidler, {K. M.} and K. Baker and K. Woods and C. Schnizlein-Bick and Oscar Cummings and R. Sidner and B. Foresman and Wilkes, {D. S.}",
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T1 - Instillation of allogeneic lung antigen-presenting cells deficient in expression of major histocompatibility complex class I or II antigens have differential effects on local cellular and humoral immunity and on pathology in recipient murine lungs

AU - Heidler, K. M.

AU - Baker, K.

AU - Woods, K.

AU - Schnizlein-Bick, C.

AU - Cummings, Oscar

AU - Sidner, R.

AU - Foresman, B.

AU - Wilkes, D. S.

PY - 2000

Y1 - 2000

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