Insulin action in pancreatic acini from streptozotocin-treated rats. I. Stimulation of protein synthesis.

Murray Korc, Y. Iwamoto, H. Sankaran, J. A. Williams, I. D. Goldfine

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Pancreatic acini were prepared from rats rendered diabetic with streptozotocin. In this tissue, insulin stimulated [3H]leucine incorporation into protein. The full effects of insulin on this function were not immediate but increased linearly with time for up to 2 h of incubation. Insulin had a detectable effect on l eucine incorporation at 50 pM, a half-maximal effect at 0.7 nM, and a maximal effect at 30 nM. Desdipeptide proinsulin was only 10% as potent as native insulin in stimulating [3H]leucine incorporation, whereas proinsulin and desoctapeptide insulin were only 1% as potent. Insulin also increased the incorporation of [3H]valine and [35S]methionine into protein but did not increase the influx of either [14C]cycloleucine or alpha-[3H]aminoisobutyric acid. These observations suggested that the increased incorporation of labeled amino acid into protein reflected stimulation of protein synthesis rather than stimulation of amino acid transport. Furthermore, insulin at 1.67 nM significantly increased the acinar cell concentration of amylase. The present findings are consistent therefore with the concept that insulin regulates pancreatic exocrine functions, including protein and enzyme synthesis.

Original languageEnglish
JournalThe American journal of physiology
Volume240
Issue number1
StatePublished - Jan 1981
Externally publishedYes

Fingerprint

Streptozocin
Insulin
Proteins
Proinsulin
Leucine
Cycloleucine
Amino Acids
Acinar Cells
Valine
Amylases
Methionine
Enzymes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Insulin action in pancreatic acini from streptozotocin-treated rats. I. Stimulation of protein synthesis. / Korc, Murray; Iwamoto, Y.; Sankaran, H.; Williams, J. A.; Goldfine, I. D.

In: The American journal of physiology, Vol. 240, No. 1, 01.1981.

Research output: Contribution to journalArticle

Korc, Murray ; Iwamoto, Y. ; Sankaran, H. ; Williams, J. A. ; Goldfine, I. D. / Insulin action in pancreatic acini from streptozotocin-treated rats. I. Stimulation of protein synthesis. In: The American journal of physiology. 1981 ; Vol. 240, No. 1.
@article{8aeb01d8282b4cd8ac084f0b182dd227,
title = "Insulin action in pancreatic acini from streptozotocin-treated rats. I. Stimulation of protein synthesis.",
abstract = "Pancreatic acini were prepared from rats rendered diabetic with streptozotocin. In this tissue, insulin stimulated [3H]leucine incorporation into protein. The full effects of insulin on this function were not immediate but increased linearly with time for up to 2 h of incubation. Insulin had a detectable effect on l eucine incorporation at 50 pM, a half-maximal effect at 0.7 nM, and a maximal effect at 30 nM. Desdipeptide proinsulin was only 10{\%} as potent as native insulin in stimulating [3H]leucine incorporation, whereas proinsulin and desoctapeptide insulin were only 1{\%} as potent. Insulin also increased the incorporation of [3H]valine and [35S]methionine into protein but did not increase the influx of either [14C]cycloleucine or alpha-[3H]aminoisobutyric acid. These observations suggested that the increased incorporation of labeled amino acid into protein reflected stimulation of protein synthesis rather than stimulation of amino acid transport. Furthermore, insulin at 1.67 nM significantly increased the acinar cell concentration of amylase. The present findings are consistent therefore with the concept that insulin regulates pancreatic exocrine functions, including protein and enzyme synthesis.",
author = "Murray Korc and Y. Iwamoto and H. Sankaran and Williams, {J. A.} and Goldfine, {I. D.}",
year = "1981",
month = "1",
language = "English",
volume = "240",
journal = "American Journal of Physiology",
issn = "0193-1857",
publisher = "American Physiological Society",
number = "1",

}

TY - JOUR

T1 - Insulin action in pancreatic acini from streptozotocin-treated rats. I. Stimulation of protein synthesis.

AU - Korc, Murray

AU - Iwamoto, Y.

AU - Sankaran, H.

AU - Williams, J. A.

AU - Goldfine, I. D.

PY - 1981/1

Y1 - 1981/1

N2 - Pancreatic acini were prepared from rats rendered diabetic with streptozotocin. In this tissue, insulin stimulated [3H]leucine incorporation into protein. The full effects of insulin on this function were not immediate but increased linearly with time for up to 2 h of incubation. Insulin had a detectable effect on l eucine incorporation at 50 pM, a half-maximal effect at 0.7 nM, and a maximal effect at 30 nM. Desdipeptide proinsulin was only 10% as potent as native insulin in stimulating [3H]leucine incorporation, whereas proinsulin and desoctapeptide insulin were only 1% as potent. Insulin also increased the incorporation of [3H]valine and [35S]methionine into protein but did not increase the influx of either [14C]cycloleucine or alpha-[3H]aminoisobutyric acid. These observations suggested that the increased incorporation of labeled amino acid into protein reflected stimulation of protein synthesis rather than stimulation of amino acid transport. Furthermore, insulin at 1.67 nM significantly increased the acinar cell concentration of amylase. The present findings are consistent therefore with the concept that insulin regulates pancreatic exocrine functions, including protein and enzyme synthesis.

AB - Pancreatic acini were prepared from rats rendered diabetic with streptozotocin. In this tissue, insulin stimulated [3H]leucine incorporation into protein. The full effects of insulin on this function were not immediate but increased linearly with time for up to 2 h of incubation. Insulin had a detectable effect on l eucine incorporation at 50 pM, a half-maximal effect at 0.7 nM, and a maximal effect at 30 nM. Desdipeptide proinsulin was only 10% as potent as native insulin in stimulating [3H]leucine incorporation, whereas proinsulin and desoctapeptide insulin were only 1% as potent. Insulin also increased the incorporation of [3H]valine and [35S]methionine into protein but did not increase the influx of either [14C]cycloleucine or alpha-[3H]aminoisobutyric acid. These observations suggested that the increased incorporation of labeled amino acid into protein reflected stimulation of protein synthesis rather than stimulation of amino acid transport. Furthermore, insulin at 1.67 nM significantly increased the acinar cell concentration of amylase. The present findings are consistent therefore with the concept that insulin regulates pancreatic exocrine functions, including protein and enzyme synthesis.

UR - http://www.scopus.com/inward/record.url?scp=0019479735&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019479735&partnerID=8YFLogxK

M3 - Article

C2 - 6161541

AN - SCOPUS:0019479735

VL - 240

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 0193-1857

IS - 1

ER -