Insulin, Leptin, and Tumoral Adipocytes Promote Murine Pancreatic Cancer Growth

Patrick B. White, Eben M. True, Kathryn M. Ziegler, Sue S. Wang, Deborah A. Swartz-Basile, Henry A. Pitt, Nicholas J. Zyromski

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

Background: Obesity accelerates development and growth of human pancreatic cancer. We recently reported similar findings in a novel murine model of pancreatic cancer in congenitally obese mice. The current experiments were designed to evaluate the effects of diet-induced obesity on pancreatic cancer growth. Methods: Thirty C57BL/6J female mice were fed either control 10% fat (n = 10) or 60% fat diet (n = 20) starting at age 6 weeks. At 11 weeks, 2.5 × 105 PAN02 murine pancreatic cancer cells were inoculated. After 6 weeks, tumors were harvested. Serum adiponectin, leptin, insulin, and glucose concentrations were measured. Tumor proliferation, apoptosis, adipocyte content, and tumor-infiltrating lymphocytes were evaluated. Results: The diet-induced obesity diet led to significant weight gain (control 21.3 ± 0.6 g; diet-induced obesity 23.1 ± 0.5 g; p = 0.03). Mice heavier than 23.1 g were considered "Overweight." Tumors grew significantly larger in overweight (1.3 ± 0.3 g) compared to lean (0.5 ± 0.2 g; p = 0.03) mice; tumor size correlated positively with body weight (R = 0.56; p < 0.02). Serum leptin (3.1 ± 0.7 vs. 1.4 ± 0.2 ng/ml) and insulin (0.5 ± 0.2 vs. 0.18 ± 0.02 ng/ml) were significantly greater in overweight mice. Tumor proliferation, apoptosis, and tumor adipocyte volume were similar. T and B lymphocytes were observed infiltrating tumors from lean and overweight mice in similar number. Conclusion: These data show that diet-induced obesity accelerates the growth of murine pancreatic cancer.

Original languageEnglish (US)
Pages (from-to)1888-1894
Number of pages7
JournalJournal of Gastrointestinal Surgery
Volume14
Issue number12
DOIs
StatePublished - Dec 1 2010

Keywords

  • Diet-induced obesity
  • Insulin
  • Leptin
  • Mouse model
  • Pancreas cancer

ASJC Scopus subject areas

  • Surgery
  • Gastroenterology

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    White, P. B., True, E. M., Ziegler, K. M., Wang, S. S., Swartz-Basile, D. A., Pitt, H. A., & Zyromski, N. J. (2010). Insulin, Leptin, and Tumoral Adipocytes Promote Murine Pancreatic Cancer Growth. Journal of Gastrointestinal Surgery, 14(12), 1888-1894. https://doi.org/10.1007/s11605-010-1349-x