Insulin, Leptin, and Tumoral Adipocytes Promote Murine Pancreatic Cancer Growth

Patrick B. White, Eben M. True, Kathryn M. Ziegler, Sue S. Wang, Deborah A. Swartz-Basile, Henry A. Pitt, Nicholas Zyromski

Research output: Contribution to journalArticle

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Abstract

Background: Obesity accelerates development and growth of human pancreatic cancer. We recently reported similar findings in a novel murine model of pancreatic cancer in congenitally obese mice. The current experiments were designed to evaluate the effects of diet-induced obesity on pancreatic cancer growth. Methods: Thirty C57BL/6J female mice were fed either control 10% fat (n = 10) or 60% fat diet (n = 20) starting at age 6 weeks. At 11 weeks, 2.5 × 105 PAN02 murine pancreatic cancer cells were inoculated. After 6 weeks, tumors were harvested. Serum adiponectin, leptin, insulin, and glucose concentrations were measured. Tumor proliferation, apoptosis, adipocyte content, and tumor-infiltrating lymphocytes were evaluated. Results: The diet-induced obesity diet led to significant weight gain (control 21.3 ± 0.6 g; diet-induced obesity 23.1 ± 0.5 g; p = 0.03). Mice heavier than 23.1 g were considered "Overweight." Tumors grew significantly larger in overweight (1.3 ± 0.3 g) compared to lean (0.5 ± 0.2 g; p = 0.03) mice; tumor size correlated positively with body weight (R = 0.56; p < 0.02). Serum leptin (3.1 ± 0.7 vs. 1.4 ± 0.2 ng/ml) and insulin (0.5 ± 0.2 vs. 0.18 ± 0.02 ng/ml) were significantly greater in overweight mice. Tumor proliferation, apoptosis, and tumor adipocyte volume were similar. T and B lymphocytes were observed infiltrating tumors from lean and overweight mice in similar number. Conclusion: These data show that diet-induced obesity accelerates the growth of murine pancreatic cancer.

Original languageEnglish
Pages (from-to)1888-1894
Number of pages7
JournalJournal of Gastrointestinal Surgery
Volume14
Issue number12
DOIs
StatePublished - Dec 2010

Fingerprint

Leptin
Pancreatic Neoplasms
Adipocytes
Insulin
Diet
Obesity
Growth
Neoplasms
Fats
Apoptosis
Tumor-Infiltrating Lymphocytes
Obese Mice
Adiponectin
Human Development
Tumor Burden
Serum
Growth and Development
Weight Gain
B-Lymphocytes
Body Weight

Keywords

  • Diet-induced obesity
  • Insulin
  • Leptin
  • Mouse model
  • Pancreas cancer

ASJC Scopus subject areas

  • Surgery
  • Gastroenterology

Cite this

White, P. B., True, E. M., Ziegler, K. M., Wang, S. S., Swartz-Basile, D. A., Pitt, H. A., & Zyromski, N. (2010). Insulin, Leptin, and Tumoral Adipocytes Promote Murine Pancreatic Cancer Growth. Journal of Gastrointestinal Surgery, 14(12), 1888-1894. https://doi.org/10.1007/s11605-010-1349-x

Insulin, Leptin, and Tumoral Adipocytes Promote Murine Pancreatic Cancer Growth. / White, Patrick B.; True, Eben M.; Ziegler, Kathryn M.; Wang, Sue S.; Swartz-Basile, Deborah A.; Pitt, Henry A.; Zyromski, Nicholas.

In: Journal of Gastrointestinal Surgery, Vol. 14, No. 12, 12.2010, p. 1888-1894.

Research output: Contribution to journalArticle

White, PB, True, EM, Ziegler, KM, Wang, SS, Swartz-Basile, DA, Pitt, HA & Zyromski, N 2010, 'Insulin, Leptin, and Tumoral Adipocytes Promote Murine Pancreatic Cancer Growth', Journal of Gastrointestinal Surgery, vol. 14, no. 12, pp. 1888-1894. https://doi.org/10.1007/s11605-010-1349-x
White PB, True EM, Ziegler KM, Wang SS, Swartz-Basile DA, Pitt HA et al. Insulin, Leptin, and Tumoral Adipocytes Promote Murine Pancreatic Cancer Growth. Journal of Gastrointestinal Surgery. 2010 Dec;14(12):1888-1894. https://doi.org/10.1007/s11605-010-1349-x
White, Patrick B. ; True, Eben M. ; Ziegler, Kathryn M. ; Wang, Sue S. ; Swartz-Basile, Deborah A. ; Pitt, Henry A. ; Zyromski, Nicholas. / Insulin, Leptin, and Tumoral Adipocytes Promote Murine Pancreatic Cancer Growth. In: Journal of Gastrointestinal Surgery. 2010 ; Vol. 14, No. 12. pp. 1888-1894.
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abstract = "Background: Obesity accelerates development and growth of human pancreatic cancer. We recently reported similar findings in a novel murine model of pancreatic cancer in congenitally obese mice. The current experiments were designed to evaluate the effects of diet-induced obesity on pancreatic cancer growth. Methods: Thirty C57BL/6J female mice were fed either control 10{\%} fat (n = 10) or 60{\%} fat diet (n = 20) starting at age 6 weeks. At 11 weeks, 2.5 × 105 PAN02 murine pancreatic cancer cells were inoculated. After 6 weeks, tumors were harvested. Serum adiponectin, leptin, insulin, and glucose concentrations were measured. Tumor proliferation, apoptosis, adipocyte content, and tumor-infiltrating lymphocytes were evaluated. Results: The diet-induced obesity diet led to significant weight gain (control 21.3 ± 0.6 g; diet-induced obesity 23.1 ± 0.5 g; p = 0.03). Mice heavier than 23.1 g were considered {"}Overweight.{"} Tumors grew significantly larger in overweight (1.3 ± 0.3 g) compared to lean (0.5 ± 0.2 g; p = 0.03) mice; tumor size correlated positively with body weight (R = 0.56; p < 0.02). Serum leptin (3.1 ± 0.7 vs. 1.4 ± 0.2 ng/ml) and insulin (0.5 ± 0.2 vs. 0.18 ± 0.02 ng/ml) were significantly greater in overweight mice. Tumor proliferation, apoptosis, and tumor adipocyte volume were similar. T and B lymphocytes were observed infiltrating tumors from lean and overweight mice in similar number. Conclusion: These data show that diet-induced obesity accelerates the growth of murine pancreatic cancer.",
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