Insulin-like growth factor I overexpression in human pancreatic cancer: Evidence for autocrine and paracrine roles

U. Bergmann, H. Funatomi, M. Yokoyama, Murray Korc, M. Korc

Research output: Contribution to journalArticle

271 Citations (Scopus)

Abstract

We assessed the potential roles of insulin-like growth factor-I (IGF-I) and the IGF-I receptor (IGF-IR) in human pancreatic cancer. IGF-I enhanced the growth of ASPC-1 and COLO-357 human pancreatic cancer cells, and this effect was significantly inhibited by a highly specific monoclonal anti-IGF- IR antibody (αIR3). Both cell lines expressed mRNA transcripts for IGF-IR, and basal cell growth was significantly reduced by an IGF-IR antisense oligodeoxynucleotide. IGF-I mRNA transcripts were not detected in either cell line or in two additional pancreatic cancer cell lines. In contrast, analysis of 12 pancreatic cancers revealed a 32-fold increase (P <0.01) in IGF-I mRNA levels by comparison with the low levels observed in the normal pancreas. By in situ hybridization, IGF-I mRNA grains were present in both the cancer cells and in the surrounding connective tissue. Six of the cancers exhibited a 4.4-fold increase in IGF-IR mRNA levels. These findings suggest that IGF-I may participate in aberrant autocrine and paracrine activation of IGF-IR in pancreatic cancer in vivo.

Original languageEnglish (US)
Pages (from-to)2007-2011
Number of pages5
JournalCancer Research
Volume55
Issue number10
StatePublished - 1995
Externally publishedYes

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IGF Type 1 Receptor
Pancreatic Neoplasms
Insulin-Like Growth Factor I
Messenger RNA
Cell Line
Oligodeoxyribonucleotides
Growth
Connective Tissue
In Situ Hybridization
Pancreas
Neoplasms
Antibodies

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Bergmann, U., Funatomi, H., Yokoyama, M., Korc, M., & Korc, M. (1995). Insulin-like growth factor I overexpression in human pancreatic cancer: Evidence for autocrine and paracrine roles. Cancer Research, 55(10), 2007-2011.

Insulin-like growth factor I overexpression in human pancreatic cancer : Evidence for autocrine and paracrine roles. / Bergmann, U.; Funatomi, H.; Yokoyama, M.; Korc, Murray; Korc, M.

In: Cancer Research, Vol. 55, No. 10, 1995, p. 2007-2011.

Research output: Contribution to journalArticle

Bergmann, U, Funatomi, H, Yokoyama, M, Korc, M & Korc, M 1995, 'Insulin-like growth factor I overexpression in human pancreatic cancer: Evidence for autocrine and paracrine roles', Cancer Research, vol. 55, no. 10, pp. 2007-2011.
Bergmann, U. ; Funatomi, H. ; Yokoyama, M. ; Korc, Murray ; Korc, M. / Insulin-like growth factor I overexpression in human pancreatic cancer : Evidence for autocrine and paracrine roles. In: Cancer Research. 1995 ; Vol. 55, No. 10. pp. 2007-2011.
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