Insulin-like growth factor I stimulates proliferation, migration, and plasminogen activator release by human retinal pigment epithelial cells

Maria B. Grant, Colleen Guay, Robert Marsh

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

The migration of retinal pigment epithelial (RPE) cells from their normal anatomic position to a new position in the vitreous cavity is a critical feature of proliferative vitreous retinopathy. To determine if insulin-like growth factor I (IGF I), which is present in the vitreous fluid of diabetics, stimulates RPE cells, we examined the effects of IGF I on the proliferation, chemotaxis, and release of plasminoqen activator by these cells. At the concentrations of IGF I tested, significant proliferation of RPE cells is seen. Significant chemotaxis of the RPE cells also is seen at all the concentrations of IGF I tested. The mean number of migrating cells per high-powered field in control studies was 43 ± 13 (x ± SEM), and for IGF I at 2.5 ng and 50 ng/ml the mean numbers of migrating cells were 96 ± 17 and 483 ± 62, respectively (P

Original languageEnglish (US)
Pages (from-to)323-335
Number of pages13
JournalCurrent Eye Research
Volume9
Issue number4
DOIs
StatePublished - 1990
Externally publishedYes

Fingerprint

Retinal Pigments
Plasminogen Activators
Insulin-Like Growth Factor I
Epithelial Cells
Chemotaxis
Cell Count

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Insulin-like growth factor I stimulates proliferation, migration, and plasminogen activator release by human retinal pigment epithelial cells. / Grant, Maria B.; Guay, Colleen; Marsh, Robert.

In: Current Eye Research, Vol. 9, No. 4, 1990, p. 323-335.

Research output: Contribution to journalArticle

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