Insulin-like growth factors in amniotic fluid

Thomas J. Merimee, M. Grant, J. E. Tyson

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

The concentrations of insulin-like growth factors I and II (IGF-I and IGF-II) in amniotic fluid were determined by specific immunoassays in 58 women. IGF-I concentrations were constant throughout gestation at approximatey 20 ng/ml; the mean IGF-II concentration was 114 ± 13 (±SE) ng/ml at the earliest period of gestation studied and remained unchanged at 26 to 33 weeks despite a greater than 50% decrease in amniotic fluid total protein. A precipitous decrease in IGF-II concentration occurred at term which was not explainable by alterations in total amniotic fluid protein concentration. The concentrations of IGF-I and IGF-II in amniotic fluid did not correlate with concentrations of these factors in maternal serum (r = 0.08 and 0.09, respectively). [125I]IGF-I and [125I]IGF-II, after incubation with amniotic fluid, bound to a 40–45 K protein (or proteins). A carrier protein of greater mol wt, as in serum, was not detected. These findings indicate that there is dynamic control of IGF in amniotic fluid during normal pregnancy.

Original languageEnglish (US)
Pages (from-to)752-755
Number of pages4
JournalJournal of Clinical Endocrinology and Metabolism
Volume59
Issue number4
DOIs
StatePublished - Oct 1984

Fingerprint

Insulin-Like Growth Factor II
Somatomedins
Amniotic Fluid
Insulin-Like Growth Factor I
Fluids
Pregnancy
Proteins
Serum
Immunoassay
Carrier Proteins
Mothers

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Insulin-like growth factors in amniotic fluid. / Merimee, Thomas J.; Grant, M.; Tyson, J. E.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 59, No. 4, 10.1984, p. 752-755.

Research output: Contribution to journalArticle

Merimee, Thomas J. ; Grant, M. ; Tyson, J. E. / Insulin-like growth factors in amniotic fluid. In: Journal of Clinical Endocrinology and Metabolism. 1984 ; Vol. 59, No. 4. pp. 752-755.
@article{57b4e04c7ca7441f8acc0b3ec1689490,
title = "Insulin-like growth factors in amniotic fluid",
abstract = "The concentrations of insulin-like growth factors I and II (IGF-I and IGF-II) in amniotic fluid were determined by specific immunoassays in 58 women. IGF-I concentrations were constant throughout gestation at approximatey 20 ng/ml; the mean IGF-II concentration was 114 ± 13 (±SE) ng/ml at the earliest period of gestation studied and remained unchanged at 26 to 33 weeks despite a greater than 50{\%} decrease in amniotic fluid total protein. A precipitous decrease in IGF-II concentration occurred at term which was not explainable by alterations in total amniotic fluid protein concentration. The concentrations of IGF-I and IGF-II in amniotic fluid did not correlate with concentrations of these factors in maternal serum (r = 0.08 and 0.09, respectively). [125I]IGF-I and [125I]IGF-II, after incubation with amniotic fluid, bound to a 40–45 K protein (or proteins). A carrier protein of greater mol wt, as in serum, was not detected. These findings indicate that there is dynamic control of IGF in amniotic fluid during normal pregnancy.",
author = "Merimee, {Thomas J.} and M. Grant and Tyson, {J. E.}",
year = "1984",
month = "10",
doi = "10.1210/jcem-59-4-752",
language = "English (US)",
volume = "59",
pages = "752--755",
journal = "Journal of Clinical Endocrinology and Metabolism",
issn = "0021-972X",
publisher = "The Endocrine Society",
number = "4",

}

TY - JOUR

T1 - Insulin-like growth factors in amniotic fluid

AU - Merimee, Thomas J.

AU - Grant, M.

AU - Tyson, J. E.

PY - 1984/10

Y1 - 1984/10

N2 - The concentrations of insulin-like growth factors I and II (IGF-I and IGF-II) in amniotic fluid were determined by specific immunoassays in 58 women. IGF-I concentrations were constant throughout gestation at approximatey 20 ng/ml; the mean IGF-II concentration was 114 ± 13 (±SE) ng/ml at the earliest period of gestation studied and remained unchanged at 26 to 33 weeks despite a greater than 50% decrease in amniotic fluid total protein. A precipitous decrease in IGF-II concentration occurred at term which was not explainable by alterations in total amniotic fluid protein concentration. The concentrations of IGF-I and IGF-II in amniotic fluid did not correlate with concentrations of these factors in maternal serum (r = 0.08 and 0.09, respectively). [125I]IGF-I and [125I]IGF-II, after incubation with amniotic fluid, bound to a 40–45 K protein (or proteins). A carrier protein of greater mol wt, as in serum, was not detected. These findings indicate that there is dynamic control of IGF in amniotic fluid during normal pregnancy.

AB - The concentrations of insulin-like growth factors I and II (IGF-I and IGF-II) in amniotic fluid were determined by specific immunoassays in 58 women. IGF-I concentrations were constant throughout gestation at approximatey 20 ng/ml; the mean IGF-II concentration was 114 ± 13 (±SE) ng/ml at the earliest period of gestation studied and remained unchanged at 26 to 33 weeks despite a greater than 50% decrease in amniotic fluid total protein. A precipitous decrease in IGF-II concentration occurred at term which was not explainable by alterations in total amniotic fluid protein concentration. The concentrations of IGF-I and IGF-II in amniotic fluid did not correlate with concentrations of these factors in maternal serum (r = 0.08 and 0.09, respectively). [125I]IGF-I and [125I]IGF-II, after incubation with amniotic fluid, bound to a 40–45 K protein (or proteins). A carrier protein of greater mol wt, as in serum, was not detected. These findings indicate that there is dynamic control of IGF in amniotic fluid during normal pregnancy.

UR - http://www.scopus.com/inward/record.url?scp=0021135111&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021135111&partnerID=8YFLogxK

U2 - 10.1210/jcem-59-4-752

DO - 10.1210/jcem-59-4-752

M3 - Article

C2 - 6384254

AN - SCOPUS:0021135111

VL - 59

SP - 752

EP - 755

JO - Journal of Clinical Endocrinology and Metabolism

JF - Journal of Clinical Endocrinology and Metabolism

SN - 0021-972X

IS - 4

ER -