Insulin receptors in isolated mouse pancreatic acini

Murray Korc, Hariharan Sankaran, K. Y. Wong, John A. Williams, Ira D. Goldfine

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Specific insulin receptors were measured in isolated mouse pancreatic acini. Scatchard analyses revealed a high affinity binding site with a Kd of 1.67 nM and a lower affinity site with a Kd of 83 nM. Binding of insulin to these receptors was rapid, one-half maximal binding occurring at 2 min and maximal binding at 30 min. Insulin stimulated the uptake of the glucose analogue 2-deoxy-D-glucose; maximum effects were detected at 1.67 μM. Insulin, in contrast, had no direct effects on alpha-aminoisobutyric acid uptake. The finding of high affinity insulin receptors in pancreatic acinar cells supports the hypothesis that insulin may directly regulate specific functions in the exocrine pancreas.

Original languageEnglish (US)
Pages (from-to)293-299
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume84
Issue number2
DOIs
StatePublished - Sep 29 1978
Externally publishedYes

Fingerprint

Insulin Receptor
Insulin
Exocrine Pancreas
Acinar Cells
Deoxyglucose
Binding Sites
Glucose

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Korc, M., Sankaran, H., Wong, K. Y., Williams, J. A., & Goldfine, I. D. (1978). Insulin receptors in isolated mouse pancreatic acini. Biochemical and Biophysical Research Communications, 84(2), 293-299. https://doi.org/10.1016/0006-291X(78)90169-9

Insulin receptors in isolated mouse pancreatic acini. / Korc, Murray; Sankaran, Hariharan; Wong, K. Y.; Williams, John A.; Goldfine, Ira D.

In: Biochemical and Biophysical Research Communications, Vol. 84, No. 2, 29.09.1978, p. 293-299.

Research output: Contribution to journalArticle

Korc, M, Sankaran, H, Wong, KY, Williams, JA & Goldfine, ID 1978, 'Insulin receptors in isolated mouse pancreatic acini', Biochemical and Biophysical Research Communications, vol. 84, no. 2, pp. 293-299. https://doi.org/10.1016/0006-291X(78)90169-9
Korc, Murray ; Sankaran, Hariharan ; Wong, K. Y. ; Williams, John A. ; Goldfine, Ira D. / Insulin receptors in isolated mouse pancreatic acini. In: Biochemical and Biophysical Research Communications. 1978 ; Vol. 84, No. 2. pp. 293-299.
@article{76e4934479594054b2ae0c66e8d252ad,
title = "Insulin receptors in isolated mouse pancreatic acini",
abstract = "Specific insulin receptors were measured in isolated mouse pancreatic acini. Scatchard analyses revealed a high affinity binding site with a Kd of 1.67 nM and a lower affinity site with a Kd of 83 nM. Binding of insulin to these receptors was rapid, one-half maximal binding occurring at 2 min and maximal binding at 30 min. Insulin stimulated the uptake of the glucose analogue 2-deoxy-D-glucose; maximum effects were detected at 1.67 μM. Insulin, in contrast, had no direct effects on alpha-aminoisobutyric acid uptake. The finding of high affinity insulin receptors in pancreatic acinar cells supports the hypothesis that insulin may directly regulate specific functions in the exocrine pancreas.",
author = "Murray Korc and Hariharan Sankaran and Wong, {K. Y.} and Williams, {John A.} and Goldfine, {Ira D.}",
year = "1978",
month = "9",
day = "29",
doi = "10.1016/0006-291X(78)90169-9",
language = "English (US)",
volume = "84",
pages = "293--299",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Insulin receptors in isolated mouse pancreatic acini

AU - Korc, Murray

AU - Sankaran, Hariharan

AU - Wong, K. Y.

AU - Williams, John A.

AU - Goldfine, Ira D.

PY - 1978/9/29

Y1 - 1978/9/29

N2 - Specific insulin receptors were measured in isolated mouse pancreatic acini. Scatchard analyses revealed a high affinity binding site with a Kd of 1.67 nM and a lower affinity site with a Kd of 83 nM. Binding of insulin to these receptors was rapid, one-half maximal binding occurring at 2 min and maximal binding at 30 min. Insulin stimulated the uptake of the glucose analogue 2-deoxy-D-glucose; maximum effects were detected at 1.67 μM. Insulin, in contrast, had no direct effects on alpha-aminoisobutyric acid uptake. The finding of high affinity insulin receptors in pancreatic acinar cells supports the hypothesis that insulin may directly regulate specific functions in the exocrine pancreas.

AB - Specific insulin receptors were measured in isolated mouse pancreatic acini. Scatchard analyses revealed a high affinity binding site with a Kd of 1.67 nM and a lower affinity site with a Kd of 83 nM. Binding of insulin to these receptors was rapid, one-half maximal binding occurring at 2 min and maximal binding at 30 min. Insulin stimulated the uptake of the glucose analogue 2-deoxy-D-glucose; maximum effects were detected at 1.67 μM. Insulin, in contrast, had no direct effects on alpha-aminoisobutyric acid uptake. The finding of high affinity insulin receptors in pancreatic acinar cells supports the hypothesis that insulin may directly regulate specific functions in the exocrine pancreas.

UR - http://www.scopus.com/inward/record.url?scp=0018083839&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018083839&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(78)90169-9

DO - 10.1016/0006-291X(78)90169-9

M3 - Article

VL - 84

SP - 293

EP - 299

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -