Insulin sensitizes β-agonist and forskolin-stimulated lipolysis to inhibition by 2′,5′-dideoxyadenosine

Yesim Polar, Elizabeth C. Coronel, Suleiman W. Bahouth, John N. Fain

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In isolated rat adipocytes incubated in the absence of insulin, 2′,5′-dideoxyadenosine blocked the increase in total adenosine 3′,5′-cyclic monophosphate (cAMP) accumulation due to β1- or β3-catecholamine agonists and forskolin without affecting their stimulation of lipolysis. The inhibition of cAMP accumulation by 2′,5′-dideoxyadenosine was not reflected in the total cytosolic cAMP-dependent protein kinase A activity, suggesting that the inhibition of cAMP occurred in cellular compartments distinct from those involved in the regulation of bulk protein kinase A activity. However, there was a good correlation between effects of lipolytic agents on cytosolic protein kinase A activity in fat cell extracts and lipolysis. Furthermore, it was possible to see an inhibition of the increase due to β-agonists in cAMP accumulation, protein kinase A activity, and lipolysis by 2′,5′-dideoxyadenosine in the presence of insulin. These data suggest that the readily measurable accumulation of cAMP seen with catecholamines in the absence of insulin is in a compartment separate from that involved in protein kinase A activation.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume270
Issue number2 39-2
StatePublished - Feb 1996
Externally publishedYes

Fingerprint

Dideoxyadenosine
Lipolysis
Colforsin
Cyclic AMP-Dependent Protein Kinases
Insulin
Adipocytes
Catecholamines
Cell Extracts
Adenosine
Rats
Chemical activation
Fats

Keywords

  • Adenosine 3′,5′-cyclic monophosphate
  • Adipocytes
  • Catecholamines
  • Protein kinase A

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology
  • Physiology (medical)

Cite this

Insulin sensitizes β-agonist and forskolin-stimulated lipolysis to inhibition by 2′,5′-dideoxyadenosine. / Polar, Yesim; Coronel, Elizabeth C.; Bahouth, Suleiman W.; Fain, John N.

In: American Journal of Physiology - Cell Physiology, Vol. 270, No. 2 39-2, 02.1996.

Research output: Contribution to journalArticle

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AU - Fain, John N.

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