Insulin-Stimulated Phosphatidylinositol 3-Kinase Activity and 2-Deoxy-D-Glucose Uptake in Rat Skeletal Muscles

Jeffrey Elmendorf, A. Damrauabney, T. R. Smith, T. S. David, J. Turinsky

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

To date there is suggestive evidence that phosphatidylinositol 3-kinase participates in insulin-stimulated glucose transport. However, its involvement in skeletal muscle, a major site of insulin-stimulated glucose disposal, has not been addressed. Therefore, we tested the effects of wortmannin, a known inhibitor of phosphatidylinositol 3-kinase, on insulin-stimulated 2-deoxyglucose uptake by rat soleus muscle in vitro. Wortmannin (1 μM) reversibly inhibited insulin-induced 2-deoxyglucose uptake in soleus muscle by 44%. Inclusion of 5 μM wortmannin in the incubation medium completely abolished the insulin-induced increment in 2-deoxyglucose uptake. In conclusion, the results are consistent with the concept that phosphatidylinositol 3-kinase plays a role in the insulin-signaling cascade linking insulin-receptor tyrosine kinase activation to glucose uptake in skeletal muscle.

Original languageEnglish (US)
Pages (from-to)1147-1153
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume208
Issue number3
DOIs
StatePublished - Mar 28 1995
Externally publishedYes

Fingerprint

Phosphatidylinositol 3-Kinase
Deoxyglucose
Muscle
Rats
Skeletal Muscle
Insulin
Glucose
Chemical activation
wortmannin

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology
  • Biophysics
  • Biochemistry

Cite this

Insulin-Stimulated Phosphatidylinositol 3-Kinase Activity and 2-Deoxy-D-Glucose Uptake in Rat Skeletal Muscles. / Elmendorf, Jeffrey; Damrauabney, A.; Smith, T. R.; David, T. S.; Turinsky, J.

In: Biochemical and Biophysical Research Communications, Vol. 208, No. 3, 28.03.1995, p. 1147-1153.

Research output: Contribution to journalArticle

@article{15e7de1aa1004a3d995e3ff5d2636e43,
title = "Insulin-Stimulated Phosphatidylinositol 3-Kinase Activity and 2-Deoxy-D-Glucose Uptake in Rat Skeletal Muscles",
abstract = "To date there is suggestive evidence that phosphatidylinositol 3-kinase participates in insulin-stimulated glucose transport. However, its involvement in skeletal muscle, a major site of insulin-stimulated glucose disposal, has not been addressed. Therefore, we tested the effects of wortmannin, a known inhibitor of phosphatidylinositol 3-kinase, on insulin-stimulated 2-deoxyglucose uptake by rat soleus muscle in vitro. Wortmannin (1 μM) reversibly inhibited insulin-induced 2-deoxyglucose uptake in soleus muscle by 44{\%}. Inclusion of 5 μM wortmannin in the incubation medium completely abolished the insulin-induced increment in 2-deoxyglucose uptake. In conclusion, the results are consistent with the concept that phosphatidylinositol 3-kinase plays a role in the insulin-signaling cascade linking insulin-receptor tyrosine kinase activation to glucose uptake in skeletal muscle.",
author = "Jeffrey Elmendorf and A. Damrauabney and Smith, {T. R.} and David, {T. S.} and J. Turinsky",
year = "1995",
month = "3",
day = "28",
doi = "10.1006/bbrc.1995.1453",
language = "English (US)",
volume = "208",
pages = "1147--1153",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Insulin-Stimulated Phosphatidylinositol 3-Kinase Activity and 2-Deoxy-D-Glucose Uptake in Rat Skeletal Muscles

AU - Elmendorf, Jeffrey

AU - Damrauabney, A.

AU - Smith, T. R.

AU - David, T. S.

AU - Turinsky, J.

PY - 1995/3/28

Y1 - 1995/3/28

N2 - To date there is suggestive evidence that phosphatidylinositol 3-kinase participates in insulin-stimulated glucose transport. However, its involvement in skeletal muscle, a major site of insulin-stimulated glucose disposal, has not been addressed. Therefore, we tested the effects of wortmannin, a known inhibitor of phosphatidylinositol 3-kinase, on insulin-stimulated 2-deoxyglucose uptake by rat soleus muscle in vitro. Wortmannin (1 μM) reversibly inhibited insulin-induced 2-deoxyglucose uptake in soleus muscle by 44%. Inclusion of 5 μM wortmannin in the incubation medium completely abolished the insulin-induced increment in 2-deoxyglucose uptake. In conclusion, the results are consistent with the concept that phosphatidylinositol 3-kinase plays a role in the insulin-signaling cascade linking insulin-receptor tyrosine kinase activation to glucose uptake in skeletal muscle.

AB - To date there is suggestive evidence that phosphatidylinositol 3-kinase participates in insulin-stimulated glucose transport. However, its involvement in skeletal muscle, a major site of insulin-stimulated glucose disposal, has not been addressed. Therefore, we tested the effects of wortmannin, a known inhibitor of phosphatidylinositol 3-kinase, on insulin-stimulated 2-deoxyglucose uptake by rat soleus muscle in vitro. Wortmannin (1 μM) reversibly inhibited insulin-induced 2-deoxyglucose uptake in soleus muscle by 44%. Inclusion of 5 μM wortmannin in the incubation medium completely abolished the insulin-induced increment in 2-deoxyglucose uptake. In conclusion, the results are consistent with the concept that phosphatidylinositol 3-kinase plays a role in the insulin-signaling cascade linking insulin-receptor tyrosine kinase activation to glucose uptake in skeletal muscle.

UR - http://www.scopus.com/inward/record.url?scp=0028933598&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028933598&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1995.1453

DO - 10.1006/bbrc.1995.1453

M3 - Article

VL - 208

SP - 1147

EP - 1153

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -