Insulin-stimulated phosphatidylinositol 3-kinase activity and 2-deoxy-d-glucose uptake in rat skeletal muscles

Jeffrey S. Elmendorf, Alice Damrauabney, Terry R. Smith, Tonya S. David, Jiri Turinsky

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

To date there is suggestive evidence that phosphatidylinositol 3-kinase participates in insulin-stimulated glucose transport. However, its involvement in skeletal muscle, a major site of insulin-stimulated glucose disposal, has not been addressed. Therefore, we tested the effects of wortmannin, a known inhibitor of phosphatidylinositol 3-kinase, on insulin-stimulated 2-deoxyglucose uptake by rat soleus muscle in vitro. Wortmannin (1 μM) reversibly inhibited insulin-induced 2-deoxyglucose uptake in soleus muscle by 44%. Inclusion of 5 μM wortmannin in the incubation medium completely abolished the insulin-induced increment in 2-deoxyglucose uptake. In conclusion, the results are consistent with the concept that phosphatidylinositol 3-kinase plays a role in the insulin-signaling cascade linking insulin-receptor tyrosine kinase activation to glucose uptake in skeletal muscle.

Original languageEnglish (US)
Pages (from-to)1147-1153
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume208
Issue number3
DOIs
StatePublished - Mar 28 1995
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Insulin-stimulated phosphatidylinositol 3-kinase activity and 2-deoxy-d-glucose uptake in rat skeletal muscles'. Together they form a unique fingerprint.

  • Cite this