Integrated Analysis of Global mRNA and Protein Expression Data in HEK293 Cells Overexpressing PRL-1

Carmen M. Dumaual, Boyd A. Steere, Chad D. Walls, Mu Wang, Zhong-Yin Zhang, Stephen K. Randall

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background:The protein tyrosine phosphatase PRL-1 represents a putative oncogene with wide-ranging cellular effects. Overexpression of PRL-1 can promote cell proliferation, survival, migration, invasion, and metastasis, but the underlying mechanisms by which it influences these processes remain poorly understood.Methodology:To increase our comprehension of PRL-1 mediated signaling events, we employed transcriptional profiling (DNA microarray) and proteomics (mass spectrometry) to perform a thorough characterization of the global molecular changes in gene expression that occur in response to stable PRL-1 overexpression in a relevant model system (HEK293).Principal Findings:Overexpression of PRL-1 led to several significant changes in the mRNA and protein expression profiles of HEK293 cells. The differentially expressed gene set was highly enriched in genes involved in cytoskeletal remodeling, integrin-mediated cell-matrix adhesion, and RNA recognition and splicing. In particular, members of the Rho signaling pathway and molecules that converge on this pathway were heavily influenced by PRL-1 overexpression, supporting observations from previous studies that link PRL-1 to the Rho GTPase signaling network. In addition, several genes not previously associated with PRL-1 were found to be significantly altered by its expression. Most notable among these were Filamin A, RhoGDIα, SPARC, hnRNPH2, and PRDX2.Conclusions and Significance:This systems-level approach sheds new light on the molecular networks underlying PRL-1 action and presents several novel directions for future, hypothesis-based studies.

Original languageEnglish
Article numbere72977
JournalPLoS One
Volume8
Issue number9
DOIs
StatePublished - Sep 3 2013

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HEK293 Cells
protein synthesis
Genes
Messenger RNA
filamin
Filamins
Cell-Matrix Junctions
RNA Splicing
protein-tyrosine-phosphatase
rho GTP-Binding Proteins
Proteins
Protein Tyrosine Phosphatases
genes
oncogenes
integrins
guanosinetriphosphatase
Cell proliferation
cells
Microarrays
Oligonucleotide Array Sequence Analysis

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Integrated Analysis of Global mRNA and Protein Expression Data in HEK293 Cells Overexpressing PRL-1. / Dumaual, Carmen M.; Steere, Boyd A.; Walls, Chad D.; Wang, Mu; Zhang, Zhong-Yin; Randall, Stephen K.

In: PLoS One, Vol. 8, No. 9, e72977, 03.09.2013.

Research output: Contribution to journalArticle

Dumaual, Carmen M. ; Steere, Boyd A. ; Walls, Chad D. ; Wang, Mu ; Zhang, Zhong-Yin ; Randall, Stephen K. / Integrated Analysis of Global mRNA and Protein Expression Data in HEK293 Cells Overexpressing PRL-1. In: PLoS One. 2013 ; Vol. 8, No. 9.
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