Interaction of β-adrenoceptor and adenosine receptor agonists on phosphorylation. Identification of target proteins in mammalian ventricles

J. Neumann, R. C. Gupta, Larry Jones, G. S. Bodor, S. Bartel, E. G. Krause, H. T. Pask, W. Schmitz, H. Scholz, A. M. Watanabe

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The influence of the adenosine derivatives (-)-N6-phenylisopropyladenosine (R-PIA, 1 μm) and 5′-N-ethylcarbox-amidoadenosine (NECA, 1 μm) on β-adrenergic stimulated (isoproterenol, 10 nm) phosphorylation of sarcolemmal (15 kDa protein), sarcoplasmic reticular (phospholamban) and myofibrillar proteins (troponin I. C-protein) was studied in isolated 32P-labeled guinea-pig ventricles. The identification of the 15 kDa protein, phospholamban, troponin I and C-protein was based on their reaction with specific antibodies. Isoproterenol increased contractile parameters (developed tension, rate of tension development, rate of relaxation) and stimulated the phosphorylation state of a 15 kDa protein (now named phospholemman), of phospholamban, troponin I and C-protein (regarded as regulatory proteins). Isoproterenol concomitantly increased myocardial cyclic AMP levels. R-PIA and NECA attenuated the effects of isoproterenol on contractile parameters as well as on the phosphorylation of the regulatory proteins without affecting cyclic AMP levels. The effects of 1 μm R-PIA and 1 μm NECA on the isoproterenol-stimulated phosporylation of regulatory proteins were blocked by the adenosine receptor antagonist 1,3-dipropyl-8-cyclopentylxanthine (DPCPX, 1 μm). Therefore, it is concluded that adenosine derivatives acting via adenosine receptors can reduce the isoproterenol-stimulated phosphorylation state of the following regulatory proteins: phospholemman, phospholamban, troponin I and C-protein.

Original languageEnglish
Pages (from-to)1655-1667
Number of pages13
JournalJournal of Molecular and Cellular Cardiology
Volume27
Issue number8
DOIs
StatePublished - 1995

Fingerprint

Purinergic P1 Receptor Agonists
Adrenergic Receptors
Phosphorylation
Isoproterenol
Troponin C
Proteins
Troponin I
Adenosine-5'-(N-ethylcarboxamide)
Cyclic AMP
Adenosine
Phenylisopropyladenosine
Purinergic P1 Receptor Antagonists
Purinergic P1 Receptors
Adrenergic Agents

Keywords

  • Adenosine
  • C-protein
  • Phospholamban
  • Phospholemman
  • Troponin I

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Cite this

Interaction of β-adrenoceptor and adenosine receptor agonists on phosphorylation. Identification of target proteins in mammalian ventricles. / Neumann, J.; Gupta, R. C.; Jones, Larry; Bodor, G. S.; Bartel, S.; Krause, E. G.; Pask, H. T.; Schmitz, W.; Scholz, H.; Watanabe, A. M.

In: Journal of Molecular and Cellular Cardiology, Vol. 27, No. 8, 1995, p. 1655-1667.

Research output: Contribution to journalArticle

Neumann, J. ; Gupta, R. C. ; Jones, Larry ; Bodor, G. S. ; Bartel, S. ; Krause, E. G. ; Pask, H. T. ; Schmitz, W. ; Scholz, H. ; Watanabe, A. M. / Interaction of β-adrenoceptor and adenosine receptor agonists on phosphorylation. Identification of target proteins in mammalian ventricles. In: Journal of Molecular and Cellular Cardiology. 1995 ; Vol. 27, No. 8. pp. 1655-1667.
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AU - Neumann, J.

AU - Gupta, R. C.

AU - Jones, Larry

AU - Bodor, G. S.

AU - Bartel, S.

AU - Krause, E. G.

AU - Pask, H. T.

AU - Schmitz, W.

AU - Scholz, H.

AU - Watanabe, A. M.

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N2 - The influence of the adenosine derivatives (-)-N6-phenylisopropyladenosine (R-PIA, 1 μm) and 5′-N-ethylcarbox-amidoadenosine (NECA, 1 μm) on β-adrenergic stimulated (isoproterenol, 10 nm) phosphorylation of sarcolemmal (15 kDa protein), sarcoplasmic reticular (phospholamban) and myofibrillar proteins (troponin I. C-protein) was studied in isolated 32P-labeled guinea-pig ventricles. The identification of the 15 kDa protein, phospholamban, troponin I and C-protein was based on their reaction with specific antibodies. Isoproterenol increased contractile parameters (developed tension, rate of tension development, rate of relaxation) and stimulated the phosphorylation state of a 15 kDa protein (now named phospholemman), of phospholamban, troponin I and C-protein (regarded as regulatory proteins). Isoproterenol concomitantly increased myocardial cyclic AMP levels. R-PIA and NECA attenuated the effects of isoproterenol on contractile parameters as well as on the phosphorylation of the regulatory proteins without affecting cyclic AMP levels. The effects of 1 μm R-PIA and 1 μm NECA on the isoproterenol-stimulated phosporylation of regulatory proteins were blocked by the adenosine receptor antagonist 1,3-dipropyl-8-cyclopentylxanthine (DPCPX, 1 μm). Therefore, it is concluded that adenosine derivatives acting via adenosine receptors can reduce the isoproterenol-stimulated phosphorylation state of the following regulatory proteins: phospholemman, phospholamban, troponin I and C-protein.

AB - The influence of the adenosine derivatives (-)-N6-phenylisopropyladenosine (R-PIA, 1 μm) and 5′-N-ethylcarbox-amidoadenosine (NECA, 1 μm) on β-adrenergic stimulated (isoproterenol, 10 nm) phosphorylation of sarcolemmal (15 kDa protein), sarcoplasmic reticular (phospholamban) and myofibrillar proteins (troponin I. C-protein) was studied in isolated 32P-labeled guinea-pig ventricles. The identification of the 15 kDa protein, phospholamban, troponin I and C-protein was based on their reaction with specific antibodies. Isoproterenol increased contractile parameters (developed tension, rate of tension development, rate of relaxation) and stimulated the phosphorylation state of a 15 kDa protein (now named phospholemman), of phospholamban, troponin I and C-protein (regarded as regulatory proteins). Isoproterenol concomitantly increased myocardial cyclic AMP levels. R-PIA and NECA attenuated the effects of isoproterenol on contractile parameters as well as on the phosphorylation of the regulatory proteins without affecting cyclic AMP levels. The effects of 1 μm R-PIA and 1 μm NECA on the isoproterenol-stimulated phosporylation of regulatory proteins were blocked by the adenosine receptor antagonist 1,3-dipropyl-8-cyclopentylxanthine (DPCPX, 1 μm). Therefore, it is concluded that adenosine derivatives acting via adenosine receptors can reduce the isoproterenol-stimulated phosphorylation state of the following regulatory proteins: phospholemman, phospholamban, troponin I and C-protein.

KW - Adenosine

KW - C-protein

KW - Phospholamban

KW - Phospholemman

KW - Troponin I

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