Interaction of synthetic proanthocyanidin dimer and trimer with bovine serum albumin and purified bean globulin fraction G-1

William E. Artz, Paul D. Bishop, A. Dunker, Edward G. Schanus, Barry G. Swanson

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

To evaluate the type of binding involved, thermodynamic analysis of the temperature dependence of proanthocyanidin binding to bovine serum albumin (BSA) and bean glycoprotein G-1 (G-1) was investigated. Binding was analyzed with tritiated proanthocyanidin by ultrafiltration to separate free ligand and protein-bound ligand. Binding constants were determined from Scatchard plots. Van't Hoff plots indicated proanthocyanidin binding to BSA was spontaneous and entropy driven. Analysis with cis-parinaric acid supported the conclusion drawn from the thermodynamic analysis that the binding of proanthocyanidin to BSA was a hydrophobic interaction. Van't Hoff plots indicated proanthocyanidin binding to native G-1 protein was also spontaneous but, in contrast to BSA, enthalpy driven. Analysis with cis-parinaric acid confirmed the hydrophilic character of proanthocyanidin binding to native G-1. Evaluation of proanthocyanidin binding to heat-denatured G-1 with cis-parinaric acid indicated hydrophobic interactions.

Original languageEnglish (US)
Pages (from-to)417-421
Number of pages5
JournalJournal of Agricultural and Food Chemistry
Volume35
Issue number3
StatePublished - 1987
Externally publishedYes

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Globulins
bovine serum albumin
Bovine Serum Albumin
proanthocyanidins
globulins
Dimers
beans
hydrophobic bonding
Hydrophobic and Hydrophilic Interactions
Thermodynamics
thermodynamics
acids
Ligands
Ultrafiltration
Entropy
ultrafiltration
enthalpy
entropy
proanthocyanidin
glycoproteins

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

Cite this

Interaction of synthetic proanthocyanidin dimer and trimer with bovine serum albumin and purified bean globulin fraction G-1. / Artz, William E.; Bishop, Paul D.; Dunker, A.; Schanus, Edward G.; Swanson, Barry G.

In: Journal of Agricultural and Food Chemistry, Vol. 35, No. 3, 1987, p. 417-421.

Research output: Contribution to journalArticle

Artz, William E. ; Bishop, Paul D. ; Dunker, A. ; Schanus, Edward G. ; Swanson, Barry G. / Interaction of synthetic proanthocyanidin dimer and trimer with bovine serum albumin and purified bean globulin fraction G-1. In: Journal of Agricultural and Food Chemistry. 1987 ; Vol. 35, No. 3. pp. 417-421.
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AU - Swanson, Barry G.

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