Interaction of the mediator head module with RNA polymerase II

Gang Cai, Yuriy L. Chaban, Tsuyoshi Imasaki, Julio A. Kovacs, Guillermo Calero, Pawel A. Penczek, Yuichiro Takagi, Francisco J. Asturias

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26 Scopus citations


Mediator, a large (21 polypeptides, MW ∼1 MDa) complex conserved throughout eukaryotes, plays an essential role in control of gene expression by conveying regulatory signals that influence the activity of the preinitiation complex. However, the precise mode of interaction between Mediator and RNA polymerase II (RNAPII), and the mechanism of regulation by Mediator remain elusive. We used cryo-electron microscopy and reconstituted in vitro transcription assays to characterize a transcriptionally-active complex including the Mediator Head module and components of a minimum preinitiation complex (RNAPII, TFIIF, TFIIB, TBP, and promoter DNA). Our results reveal how the Head interacts with RNAPII, affecting its conformation and function.

Original languageEnglish (US)
Pages (from-to)899-910
Number of pages12
Issue number5
StatePublished - May 9 2012

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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    Cai, G., Chaban, Y. L., Imasaki, T., Kovacs, J. A., Calero, G., Penczek, P. A., Takagi, Y., & Asturias, F. J. (2012). Interaction of the mediator head module with RNA polymerase II. Structure, 20(5), 899-910.