Interaction of the mediator head module with RNA polymerase II

Gang Cai, Yuriy L. Chaban, Tsuyoshi Imasaki, Julio A. Kovacs, Guillermo Calero, Pawel A. Penczek, Yuichiro Takagi, Francisco J. Asturias

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Mediator, a large (21 polypeptides, MW ∼1 MDa) complex conserved throughout eukaryotes, plays an essential role in control of gene expression by conveying regulatory signals that influence the activity of the preinitiation complex. However, the precise mode of interaction between Mediator and RNA polymerase II (RNAPII), and the mechanism of regulation by Mediator remain elusive. We used cryo-electron microscopy and reconstituted in vitro transcription assays to characterize a transcriptionally-active complex including the Mediator Head module and components of a minimum preinitiation complex (RNAPII, TFIIF, TFIIB, TBP, and promoter DNA). Our results reveal how the Head interacts with RNAPII, affecting its conformation and function.

Original languageEnglish
Pages (from-to)899-910
Number of pages12
JournalStructure
Volume20
Issue number5
DOIs
StatePublished - May 9 2012

Fingerprint

RNA Polymerase II
Head
Mediator Complex
Transcription Factor TFIIB
Cryoelectron Microscopy
Eukaryota
Gene Expression
Peptides
DNA

ASJC Scopus subject areas

  • Molecular Biology
  • Structural Biology

Cite this

Cai, G., Chaban, Y. L., Imasaki, T., Kovacs, J. A., Calero, G., Penczek, P. A., ... Asturias, F. J. (2012). Interaction of the mediator head module with RNA polymerase II. Structure, 20(5), 899-910. https://doi.org/10.1016/j.str.2012.02.023

Interaction of the mediator head module with RNA polymerase II. / Cai, Gang; Chaban, Yuriy L.; Imasaki, Tsuyoshi; Kovacs, Julio A.; Calero, Guillermo; Penczek, Pawel A.; Takagi, Yuichiro; Asturias, Francisco J.

In: Structure, Vol. 20, No. 5, 09.05.2012, p. 899-910.

Research output: Contribution to journalArticle

Cai, G, Chaban, YL, Imasaki, T, Kovacs, JA, Calero, G, Penczek, PA, Takagi, Y & Asturias, FJ 2012, 'Interaction of the mediator head module with RNA polymerase II', Structure, vol. 20, no. 5, pp. 899-910. https://doi.org/10.1016/j.str.2012.02.023
Cai G, Chaban YL, Imasaki T, Kovacs JA, Calero G, Penczek PA et al. Interaction of the mediator head module with RNA polymerase II. Structure. 2012 May 9;20(5):899-910. https://doi.org/10.1016/j.str.2012.02.023
Cai, Gang ; Chaban, Yuriy L. ; Imasaki, Tsuyoshi ; Kovacs, Julio A. ; Calero, Guillermo ; Penczek, Pawel A. ; Takagi, Yuichiro ; Asturias, Francisco J. / Interaction of the mediator head module with RNA polymerase II. In: Structure. 2012 ; Vol. 20, No. 5. pp. 899-910.
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