Interaction of the Nck adapter protein with p21-activated kinase (PAK1)

Gary M. Bokoch, Yan Wang, Benjamin P. Bohl, Mary Ann Sells, Lawrence Quilliam, Ulla G. Knaus

Research output: Contribution to journalArticle

256 Citations (Scopus)

Abstract

The p21-activated kinases (PAKs) link G protein-coupled receptors and growth factor receptors (S. Dharmawardhane, R. H. Daniels, and G. M. Bokoch, submitted for publication) to activation of MAP kinase cascades and to cytoskeletal reorganization (M. A. Sells, U. G. Knaus, D. Ambrose, S. Bagrodia, G. M. Bokoch, and J. Chernoff, submitted for publication). The proteins that interact with PAK to mediate its cellular effects and to couple it to upstream receptors are unknown. We describe here a specific interaction of the Nck adapter molecule with PAK1 both in vitro and in vivo. PAK1 and Nck associate in COS-7 and Swiss 3T3 cells constitutively, but this interaction is strengthened upon platelet-derived growth factor receptor stimulation. We show that Nck binds to PAK1 through its second Src homology 3 (SH3) domain, while PAK1 interacts with Nck via the first proline-rich SH3 binding motif at its amino terminus. The interaction of active PAK1 with Nck leads to the phosphorylation of Nck at multiple sites. Association of Nck with PAK1 may serve to link this important regulatory kinase to cell activation by growth factor receptors.

Original languageEnglish
Pages (from-to)25746-25749
Number of pages4
JournalJournal of Biological Chemistry
Volume271
Issue number42
DOIs
StatePublished - 1996

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p21-Activated Kinases
Growth Factor Receptors
Publications
Phosphotransferases
Chemical activation
Swiss 3T3 Cells
Platelet-Derived Growth Factor Receptors
Phosphorylation
src Homology Domains
MAP Kinase Signaling System
G-Protein-Coupled Receptors
Proline
Cell Communication
Proteins
Molecules
Nck protein
In Vitro Techniques
link protein

ASJC Scopus subject areas

  • Biochemistry

Cite this

Interaction of the Nck adapter protein with p21-activated kinase (PAK1). / Bokoch, Gary M.; Wang, Yan; Bohl, Benjamin P.; Sells, Mary Ann; Quilliam, Lawrence; Knaus, Ulla G.

In: Journal of Biological Chemistry, Vol. 271, No. 42, 1996, p. 25746-25749.

Research output: Contribution to journalArticle

Bokoch, Gary M. ; Wang, Yan ; Bohl, Benjamin P. ; Sells, Mary Ann ; Quilliam, Lawrence ; Knaus, Ulla G. / Interaction of the Nck adapter protein with p21-activated kinase (PAK1). In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 42. pp. 25746-25749.
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