Interactions between purified murine colony-stimulating factors (natural CSF-1, recombinant GM-CSF, and recombinant IL-3) on the in vitro proliferation of purified murine granulocyte-macrophage progenitor cells

D. E. Williams, J. E. Straneva, S. Cooper, R. K. Shadduck, A. Waheed, S. Gillis, D. Urdal, Hal Broxmeyer

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Abstract

Purified preparations of natural CSF-1 (nCSF-1), recombinant GM-CSF (rGM-CSF), and recombinant IL-3 (rIL-3), alone and in combination, were investigated for their proliferative effects on highly enriched murine granulocyte-macrophage progenitor cells (CFU-GM). These CFU-GM had cloning efficiencies of 62%-95% in the presence of 10% (vol/vol) pokweeed mitogen-stimulated spleen cell-conditioned medium, and contained few, if any (≤3%), contaminating morphologically recognizable monocytes or lymphocytes. The combination of low concentrations of nCSF-1 plus rIL-3, or nCSF-1 plus rGM-CSF, increased colony number greater than additively compared to the sum of colony formation with each factor alone, whereas total aggregate (colony plus cluster) number increased additively. At plateau concentrations, the previous CSF combinations increased colony number additively. Colony size was increased when nCSF-1 plus either rGM-CSF or rIL-3 were added simultaneously at either low or plateau concentrations, when compared to the size of colonies with any of the CSFs alone. Addition of rGM-CSF plus rIL-3 demonstrated no cooperative proliferative effect on neither colony number or size. It is likely that these effects are mediated at the progenitor cell level and do not require accessory cell participation.

Original languageEnglish
Pages (from-to)1007-1012
Number of pages6
JournalExperimental Hematology
Volume15
Issue number10
StatePublished - 1987

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Colony-Stimulating Factors
Granulocyte-Macrophage Progenitor Cells
Macrophage Colony-Stimulating Factor
Interleukin-3
Granulocyte-Macrophage Colony-Stimulating Factor
Conditioned Culture Medium
Mitogens
Organism Cloning
Monocytes
Stem Cells
Spleen
Lymphocytes
In Vitro Techniques

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Interactions between purified murine colony-stimulating factors (natural CSF-1, recombinant GM-CSF, and recombinant IL-3) on the in vitro proliferation of purified murine granulocyte-macrophage progenitor cells. / Williams, D. E.; Straneva, J. E.; Cooper, S.; Shadduck, R. K.; Waheed, A.; Gillis, S.; Urdal, D.; Broxmeyer, Hal.

In: Experimental Hematology, Vol. 15, No. 10, 1987, p. 1007-1012.

Research output: Contribution to journalArticle

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abstract = "Purified preparations of natural CSF-1 (nCSF-1), recombinant GM-CSF (rGM-CSF), and recombinant IL-3 (rIL-3), alone and in combination, were investigated for their proliferative effects on highly enriched murine granulocyte-macrophage progenitor cells (CFU-GM). These CFU-GM had cloning efficiencies of 62{\%}-95{\%} in the presence of 10{\%} (vol/vol) pokweeed mitogen-stimulated spleen cell-conditioned medium, and contained few, if any (≤3{\%}), contaminating morphologically recognizable monocytes or lymphocytes. The combination of low concentrations of nCSF-1 plus rIL-3, or nCSF-1 plus rGM-CSF, increased colony number greater than additively compared to the sum of colony formation with each factor alone, whereas total aggregate (colony plus cluster) number increased additively. At plateau concentrations, the previous CSF combinations increased colony number additively. Colony size was increased when nCSF-1 plus either rGM-CSF or rIL-3 were added simultaneously at either low or plateau concentrations, when compared to the size of colonies with any of the CSFs alone. Addition of rGM-CSF plus rIL-3 demonstrated no cooperative proliferative effect on neither colony number or size. It is likely that these effects are mediated at the progenitor cell level and do not require accessory cell participation.",
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T1 - Interactions between purified murine colony-stimulating factors (natural CSF-1, recombinant GM-CSF, and recombinant IL-3) on the in vitro proliferation of purified murine granulocyte-macrophage progenitor cells

AU - Williams, D. E.

AU - Straneva, J. E.

AU - Cooper, S.

AU - Shadduck, R. K.

AU - Waheed, A.

AU - Gillis, S.

AU - Urdal, D.

AU - Broxmeyer, Hal

PY - 1987

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N2 - Purified preparations of natural CSF-1 (nCSF-1), recombinant GM-CSF (rGM-CSF), and recombinant IL-3 (rIL-3), alone and in combination, were investigated for their proliferative effects on highly enriched murine granulocyte-macrophage progenitor cells (CFU-GM). These CFU-GM had cloning efficiencies of 62%-95% in the presence of 10% (vol/vol) pokweeed mitogen-stimulated spleen cell-conditioned medium, and contained few, if any (≤3%), contaminating morphologically recognizable monocytes or lymphocytes. The combination of low concentrations of nCSF-1 plus rIL-3, or nCSF-1 plus rGM-CSF, increased colony number greater than additively compared to the sum of colony formation with each factor alone, whereas total aggregate (colony plus cluster) number increased additively. At plateau concentrations, the previous CSF combinations increased colony number additively. Colony size was increased when nCSF-1 plus either rGM-CSF or rIL-3 were added simultaneously at either low or plateau concentrations, when compared to the size of colonies with any of the CSFs alone. Addition of rGM-CSF plus rIL-3 demonstrated no cooperative proliferative effect on neither colony number or size. It is likely that these effects are mediated at the progenitor cell level and do not require accessory cell participation.

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