Interferon- γ responses to Plasmodium falciparum vaccine candidate antigens decrease in the absence of malaria transmission

Cyrus Ayieko, Bilha S. Ogola, Lyticia Ochola, Gideon A M Ngwena, George Ayodo, James S. Hodges, Gregory S. Noland, Chandy John

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background. Malaria elimination campaigns are planned or active in many countries. The effects of malaria elimination on immune responses such as antigen-specific IFN- γ responses are not well characterized. Methods. IFN- γ responses to the P. falciparum antigens circumsporozoite protein, liver stage antigen-1, thrombospondin-related adhesive protein, apical membrane antigen-1, MB2, and merozoite surface protein-1 were tested by ELISA in 243 individuals in highland Kenya in April 2008, October 2008, and April 2009, after a one-year period of interrupted malaria transmission from April 2007 to March 2008. Results. While one individual (0.4%) tested positive for P. falciparum byPCRinOctober 2008 and another two (0.9%) tested positive in April 2009, no clinical malaria cases were detected during weekly visits. Levels of IFN- γto all antigens decreased significantly from April 2008 to April 2009 (all P < 0.001). Discussion. Naturally acquired IFN- γ responses to P. falciparum antigensare shortlived in the absence of repeated P. falciparum infection. Even short periods of malaria interruption may significantly decrease IFN- γ responses to P. falciparum antigens.

Original languageEnglish (US)
Article numbere2855
JournalPeerJ
Volume2017
Issue number1
DOIs
StatePublished - 2017

Fingerprint

Plasmodium falciparum
interferons
malaria
Interferons
Malaria
Vaccines
vaccines
antigens
Antigens
Merozoite Surface Protein 1
Thrombospondin 1
liver protein
merozoites
Kenya
surface proteins
adhesives
Adhesives
Liver
Membrane Proteins
highlands

Keywords

  • Highland Kenya
  • Interferon gamma
  • Malaria
  • Plasmodium falciparum

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Interferon- γ responses to Plasmodium falciparum vaccine candidate antigens decrease in the absence of malaria transmission. / Ayieko, Cyrus; Ogola, Bilha S.; Ochola, Lyticia; Ngwena, Gideon A M; Ayodo, George; Hodges, James S.; Noland, Gregory S.; John, Chandy.

In: PeerJ, Vol. 2017, No. 1, e2855, 2017.

Research output: Contribution to journalArticle

Ayieko, C, Ogola, BS, Ochola, L, Ngwena, GAM, Ayodo, G, Hodges, JS, Noland, GS & John, C 2017, 'Interferon- γ responses to Plasmodium falciparum vaccine candidate antigens decrease in the absence of malaria transmission', PeerJ, vol. 2017, no. 1, e2855. https://doi.org/10.7717/peerj.2855
Ayieko, Cyrus ; Ogola, Bilha S. ; Ochola, Lyticia ; Ngwena, Gideon A M ; Ayodo, George ; Hodges, James S. ; Noland, Gregory S. ; John, Chandy. / Interferon- γ responses to Plasmodium falciparum vaccine candidate antigens decrease in the absence of malaria transmission. In: PeerJ. 2017 ; Vol. 2017, No. 1.
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abstract = "Background. Malaria elimination campaigns are planned or active in many countries. The effects of malaria elimination on immune responses such as antigen-specific IFN- γ responses are not well characterized. Methods. IFN- γ responses to the P. falciparum antigens circumsporozoite protein, liver stage antigen-1, thrombospondin-related adhesive protein, apical membrane antigen-1, MB2, and merozoite surface protein-1 were tested by ELISA in 243 individuals in highland Kenya in April 2008, October 2008, and April 2009, after a one-year period of interrupted malaria transmission from April 2007 to March 2008. Results. While one individual (0.4{\%}) tested positive for P. falciparum byPCRinOctober 2008 and another two (0.9{\%}) tested positive in April 2009, no clinical malaria cases were detected during weekly visits. Levels of IFN- γto all antigens decreased significantly from April 2008 to April 2009 (all P < 0.001). Discussion. Naturally acquired IFN- γ responses to P. falciparum antigensare shortlived in the absence of repeated P. falciparum infection. Even short periods of malaria interruption may significantly decrease IFN- γ responses to P. falciparum antigens.",
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AB - Background. Malaria elimination campaigns are planned or active in many countries. The effects of malaria elimination on immune responses such as antigen-specific IFN- γ responses are not well characterized. Methods. IFN- γ responses to the P. falciparum antigens circumsporozoite protein, liver stage antigen-1, thrombospondin-related adhesive protein, apical membrane antigen-1, MB2, and merozoite surface protein-1 were tested by ELISA in 243 individuals in highland Kenya in April 2008, October 2008, and April 2009, after a one-year period of interrupted malaria transmission from April 2007 to March 2008. Results. While one individual (0.4%) tested positive for P. falciparum byPCRinOctober 2008 and another two (0.9%) tested positive in April 2009, no clinical malaria cases were detected during weekly visits. Levels of IFN- γto all antigens decreased significantly from April 2008 to April 2009 (all P < 0.001). Discussion. Naturally acquired IFN- γ responses to P. falciparum antigensare shortlived in the absence of repeated P. falciparum infection. Even short periods of malaria interruption may significantly decrease IFN- γ responses to P. falciparum antigens.

KW - Highland Kenya

KW - Interferon gamma

KW - Malaria

KW - Plasmodium falciparum

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