Interleukin-10 in combination with M-CSF and IL-4 contributes to development of the rare population of CD14+CD16++ cells derived from human monocytes

Geling Li, Giao Hangoc, Hal Broxmeyer

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Peripheral blood CD14+CD16++ monocytes (Mo) are a rare Mo subpopulation known to undergo expansion in various diseases. We show here that IL-10 in the presence of M-CSF and IL-4 triggers the generation of CD14+CD16++ cells from highly purified human cord blood (CB) and adult blood Mo. CB Mo were more sensitive to this cytokine combination than adult Mo. IL-10-induced CD14+CD16++ cells that expressed dendritic cell markers: CD80, CD86, HLA-DR, and CD83 and initiated significantly decreased allogeneic mixed lymphocyte reactions (MLRs). Blockage of CD86, but not CD80, further down-modulated MLRs induced by CD14 +CD16++cells. CD14+CD16++ cells had similar features to CD14+CD16++ Mo in that they expressed increased level of CCR5, efficiently produced TNF-α, and displayed higher MLR than CD14+CD16- Mo. Together, these results demonstrate that M-CSF, IL-4, and IL-10 drive Mo into CD14+CD16 ++ cells similar to those identified in vivo, and CB Mo, due to their increased responsiveness, may be a useful starting cell source to study differentiation of CD14+CD16++ cells.

Original languageEnglish
Pages (from-to)637-643
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume322
Issue number2
DOIs
StatePublished - Sep 17 2004
Externally publishedYes

Fingerprint

Macrophage Colony-Stimulating Factor
Interleukin-4
Interleukin-10
Monocytes
Blood
Lymphocytes
Population
Mixed Lymphocyte Culture Test
Fetal Blood
HLA-DR Antigens
Cytokines
Dendritic Cells

Keywords

  • Cord blood
  • Dendritic cell and CCR5
  • Interleukin-10
  • Monocyte

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

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title = "Interleukin-10 in combination with M-CSF and IL-4 contributes to development of the rare population of CD14+CD16++ cells derived from human monocytes",
abstract = "Peripheral blood CD14+CD16++ monocytes (Mo) are a rare Mo subpopulation known to undergo expansion in various diseases. We show here that IL-10 in the presence of M-CSF and IL-4 triggers the generation of CD14+CD16++ cells from highly purified human cord blood (CB) and adult blood Mo. CB Mo were more sensitive to this cytokine combination than adult Mo. IL-10-induced CD14+CD16++ cells that expressed dendritic cell markers: CD80, CD86, HLA-DR, and CD83 and initiated significantly decreased allogeneic mixed lymphocyte reactions (MLRs). Blockage of CD86, but not CD80, further down-modulated MLRs induced by CD14 +CD16++cells. CD14+CD16++ cells had similar features to CD14+CD16++ Mo in that they expressed increased level of CCR5, efficiently produced TNF-α, and displayed higher MLR than CD14+CD16- Mo. Together, these results demonstrate that M-CSF, IL-4, and IL-10 drive Mo into CD14+CD16 ++ cells similar to those identified in vivo, and CB Mo, due to their increased responsiveness, may be a useful starting cell source to study differentiation of CD14+CD16++ cells.",
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AU - Broxmeyer, Hal

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N2 - Peripheral blood CD14+CD16++ monocytes (Mo) are a rare Mo subpopulation known to undergo expansion in various diseases. We show here that IL-10 in the presence of M-CSF and IL-4 triggers the generation of CD14+CD16++ cells from highly purified human cord blood (CB) and adult blood Mo. CB Mo were more sensitive to this cytokine combination than adult Mo. IL-10-induced CD14+CD16++ cells that expressed dendritic cell markers: CD80, CD86, HLA-DR, and CD83 and initiated significantly decreased allogeneic mixed lymphocyte reactions (MLRs). Blockage of CD86, but not CD80, further down-modulated MLRs induced by CD14 +CD16++cells. CD14+CD16++ cells had similar features to CD14+CD16++ Mo in that they expressed increased level of CCR5, efficiently produced TNF-α, and displayed higher MLR than CD14+CD16- Mo. Together, these results demonstrate that M-CSF, IL-4, and IL-10 drive Mo into CD14+CD16 ++ cells similar to those identified in vivo, and CB Mo, due to their increased responsiveness, may be a useful starting cell source to study differentiation of CD14+CD16++ cells.

AB - Peripheral blood CD14+CD16++ monocytes (Mo) are a rare Mo subpopulation known to undergo expansion in various diseases. We show here that IL-10 in the presence of M-CSF and IL-4 triggers the generation of CD14+CD16++ cells from highly purified human cord blood (CB) and adult blood Mo. CB Mo were more sensitive to this cytokine combination than adult Mo. IL-10-induced CD14+CD16++ cells that expressed dendritic cell markers: CD80, CD86, HLA-DR, and CD83 and initiated significantly decreased allogeneic mixed lymphocyte reactions (MLRs). Blockage of CD86, but not CD80, further down-modulated MLRs induced by CD14 +CD16++cells. CD14+CD16++ cells had similar features to CD14+CD16++ Mo in that they expressed increased level of CCR5, efficiently produced TNF-α, and displayed higher MLR than CD14+CD16- Mo. Together, these results demonstrate that M-CSF, IL-4, and IL-10 drive Mo into CD14+CD16 ++ cells similar to those identified in vivo, and CB Mo, due to their increased responsiveness, may be a useful starting cell source to study differentiation of CD14+CD16++ cells.

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