Interleukin-11 enhancement of VLA-5 mediated adhesion of CD34+ cells from cord blood to fibronectin is associated with the PI-3 kinase pathway

Li Sheng Wang, Hong Jun Liu, Hal E. Broxmeyer, Li Lu

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Adhesion is required for cell growth, differentiation, survival, and function. Cell adhesion is mediated by a structurally diverse group of plasma membrane receptors, each exhibiting specialized ligand-binding properties that are needed for specific tasks. Integrin-mediated adhesion is important for hematopoietic stem (HSC)/progenitor (HPC) cell survival and may prevent programmed cell death. Interleukin (IL)-11, a multi-functional cytokine secreted by the bone marrow environment, plays an important role in regulating growth and differentiation of HSCs/HPCs. In this report, we demonstrate that IL-II enhanced adhesion of freshly isolated and 3 day-expanded CD34+ cells to immobilized fibronectin. The expression of very late antigen (VLA)-4 and VLA-5 integrins was detected on CD34+ cells. CD34+ cells also expressed a-chain and gp130 subunits of the IL-11 receptor (R). Enhanced adhesion by IL-11 was mediated via activation of VLA-5 integrins, since this action could be blocked by monoclonal antibodies against β1 and α5, but not α4, integrins. Addition of phosphatidylinositol (PI) -3 kinase inhibitors blocked IL-11 enhanced adhesion of CD34+ cells to fibronectin. The results suggest that this enhanced adhesion is associated with the PI-3 kinase pathway, an inside-out signaling pathway.

Original languageEnglish (US)
Pages (from-to)331-337
Number of pages7
JournalIn Vivo
Volume14
Issue number2
StatePublished - Mar 2000

Keywords

  • Adhesion
  • Cord blood
  • Progenitorcells

ASJC Scopus subject areas

  • Medicine(all)

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