Interleukin-11 inhibits adipogenesis and stimulates myelopoiesis in human long-term marrow cultures

D. C. Keller, X. X. Du, Edward Srour, R. Hoffman, D. A. Williams

Research output: Contribution to journalArticle

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Abstract

Interleukin-11 (IL-11) is a bone marrow (BM) stromal-derived growth factor that has been shown to stimulate murine myeloid and lymphoid cells both in vitro and in vivo and to inhibit adipogenesis in a murine fibroblast cell line. We have studied the effects of IL-11 on highly purified human BM stem and progenitor cells and on human long-term marrow cultures (LTMC). Adipocyte differentiation is an integral component of murine and human LTMC. IL-11 stimulates myeloid growth as a single cytokine when added to highly enriched CD34+, HLA-DR+ bone marrow cells. IL-11 stimulated no growth in the more primitive CD34+, HLA-DR- population even in the presence of additional cytokines. IL-11 addition to human LTMC resulted in the expansion of myeloid and mixed, but not erythroid, progenitor populations. IL-11 dramatically increased the adherent cell populations, including both stromal cells and macrophages. Treated cultures also showed marked inhibition of fat accumulation in the adherent cells due in part to a block in the differentiation of preadipocytes to adipocytes, as shown by RNA analysis using adipocyte-specific markers. These data show that IL-11 stimulates a more differentiated, although multipotential, progenitor cell in human BM and that LTMC provide a useful model for studying the effects of this cytokine in the context of the hematopoietic microenvironment.

Original languageEnglish (US)
Pages (from-to)1428-1435
Number of pages8
JournalBlood
Volume82
Issue number5
StatePublished - Sep 1 1993

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Myelopoiesis
Interleukin-11
Adipogenesis
Bone Marrow
Bone
Adipocytes
Stem Cells
Cells
HLA-DR Antigens
Cytokines
Bone Marrow Cells
Population
Macrophages
Myeloid Cells
Fibroblasts
Stromal Cells
Growth
Cell culture
Intercellular Signaling Peptides and Proteins
Fats

ASJC Scopus subject areas

  • Hematology

Cite this

Keller, D. C., Du, X. X., Srour, E., Hoffman, R., & Williams, D. A. (1993). Interleukin-11 inhibits adipogenesis and stimulates myelopoiesis in human long-term marrow cultures. Blood, 82(5), 1428-1435.

Interleukin-11 inhibits adipogenesis and stimulates myelopoiesis in human long-term marrow cultures. / Keller, D. C.; Du, X. X.; Srour, Edward; Hoffman, R.; Williams, D. A.

In: Blood, Vol. 82, No. 5, 01.09.1993, p. 1428-1435.

Research output: Contribution to journalArticle

Keller, DC, Du, XX, Srour, E, Hoffman, R & Williams, DA 1993, 'Interleukin-11 inhibits adipogenesis and stimulates myelopoiesis in human long-term marrow cultures', Blood, vol. 82, no. 5, pp. 1428-1435.
Keller, D. C. ; Du, X. X. ; Srour, Edward ; Hoffman, R. ; Williams, D. A. / Interleukin-11 inhibits adipogenesis and stimulates myelopoiesis in human long-term marrow cultures. In: Blood. 1993 ; Vol. 82, No. 5. pp. 1428-1435.
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