Interleukin-9 Is Required for Allergic Airway Inflammation Mediated by the Cytokine TSLP

Weiguo Yao, Yanlu Zhang, Rukhsana Jabeen, Evelyn T. Nguyen, David S. Wilkes, Robert Tepper, Mark Kaplan, Baohua Zhou

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

Thymic stromal lymphopoietin (TSLP) is an epithelial cell-derived cytokine important for the initiation and development of T helper (Th2) cell-mediated allergic inflammation. In this study, we identified a positive association between interleukin-9 (IL-9) and TSLP concentration in the serum of infants with atopic dermatitis. In primary cell cultures, the addition of TSLP led to an increase in IL-9 production from human and mouse Th9 cells, and induced an increase in signal transducer and activator of transcription 5 (STAT5) activation and binding to the . Il9 promoter. In vivo, use of an adoptive transfer model demonstrated that TSLP promoted IL-9-dependent, Th9 cell-induced allergic inflammation by acting directly on T cells. Moreover, transgenic expression of TSLP in the lung stimulated IL-9 production in vivo, and anti-IL-9 treatment attenuated TSLP-induced airway inflammation. Together, our results demonstrate that TSLP promotes Th9 cell differentiation and function and define a requirement for IL-9 in TSLP-induced allergic inflammation.

Original languageEnglish
Pages (from-to)360-372
Number of pages13
JournalImmunity
Volume38
Issue number2
DOIs
StatePublished - Feb 21 2013

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Interleukin-9
Cytokines
Inflammation
STAT5 Transcription Factor
Th2 Cells
Primary Cell Culture
Adoptive Transfer
thymic stromal lymphopoietin
Atopic Dermatitis
Helper-Inducer T-Lymphocytes
Transcriptional Activation
Cell Differentiation
Epithelial Cells
T-Lymphocytes
Lung

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases
  • Immunology

Cite this

Interleukin-9 Is Required for Allergic Airway Inflammation Mediated by the Cytokine TSLP. / Yao, Weiguo; Zhang, Yanlu; Jabeen, Rukhsana; Nguyen, Evelyn T.; Wilkes, David S.; Tepper, Robert; Kaplan, Mark; Zhou, Baohua.

In: Immunity, Vol. 38, No. 2, 21.02.2013, p. 360-372.

Research output: Contribution to journalArticle

Yao, Weiguo ; Zhang, Yanlu ; Jabeen, Rukhsana ; Nguyen, Evelyn T. ; Wilkes, David S. ; Tepper, Robert ; Kaplan, Mark ; Zhou, Baohua. / Interleukin-9 Is Required for Allergic Airway Inflammation Mediated by the Cytokine TSLP. In: Immunity. 2013 ; Vol. 38, No. 2. pp. 360-372.
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