Interspecies comparative genomic hybridization (I-CGH): A new twist to study animal tumor models

Sivakumar Jaikumar, Zhengping Zhuang, Poonam Mannan, Alexander O. Vortmeyer, Makoto Furuta, Rob Dickerman, Julia Bedanova, Russell R. Lonser, Stuart Walbridge, Robert J. Weil, Victor V. Lobanenkov, Edward H. Oldfield, Svetlana D. Pack

Research output: Contribution to journalArticle

Abstract

Animal models of human diseases are widely used to address questions of tumor development. Selection of a particular animal model depends upon a variety of factors, among them: animal cost, species lifespan and hardiness; availability of biomolecular and genetic tools for that species; and evolutionary distance from humans. In spite of the growth in genomic data in the past several years, many animal models cannot yet be studied extensively due to gaps in genetic mapping, sequencing and functional analyses. Thus, alternative molecular genetic approaches are needed. We have designed an interspecies comparative genomic hybridization approach to analyze genetic changes in radiation-induced brain tumors in the non-human primate, Macaca mulatta. Using homologies between the primate and human genomes, we adapted widely-available CGH techniques to generate cytogenetic profiles of malignant gliomas in four monkey tumors. Losses and gains were projected onto the corresponding homologous chromosomal regions in the human genome, thus directly translating the status of the monkey gliomas into human gene content. This represents a novel method of comparative interspecies cytogenetic mapping that permits simultaneous analysis of genomic imbalances of unknown sequences in disparate species and correlation with potential or known human disease-related genes.

Original languageEnglish (US)
Pages (from-to)836-842
Number of pages7
JournalCell Cycle
Volume6
Issue number7
DOIs
StatePublished - Apr 1 2007
Externally publishedYes

Keywords

  • Brain
  • Glioblastoma multiforme
  • Induced tumor
  • Model
  • Primate
  • Radiation therapy
  • Universal CGH

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Interspecies comparative genomic hybridization (I-CGH): A new twist to study animal tumor models'. Together they form a unique fingerprint.

  • Cite this

    Jaikumar, S., Zhuang, Z., Mannan, P., Vortmeyer, A. O., Furuta, M., Dickerman, R., Bedanova, J., Lonser, R. R., Walbridge, S., Weil, R. J., Lobanenkov, V. V., Oldfield, E. H., & Pack, S. D. (2007). Interspecies comparative genomic hybridization (I-CGH): A new twist to study animal tumor models. Cell Cycle, 6(7), 836-842. https://doi.org/10.4161/cc.6.7.4062