Interstitial purine metabolites in hearts with LV remodeling

Andrey V. Gourine, Qingsong Hu, Paul R. Sander, Aleksandr I. Kuzmin, Nadia Hanafy, Svetlana A. Davydova, Dmitry V. Zaretsky, Jianyi Zhang

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

The myocardial ATP concentration is significantly decreased in failing hearts, which may be related to the progressive loss of the myocardial total adenine nucleotide pool. The total myocardial interstitial purine metabolites (IPM) in the dialysate of interstitial fluid could reflect the tissue ATP depletion. In rats, postmyocardial infarction (MI) left ventricular (LV) remodeling was induced by ligation of the coronary artery. Cardiac microdialysis was employed to assess changes of IPM in response to graded β-adrenergic stimulation with isoproterenol (Iso) in myocardium of hearts with post-MI LV remodeling (MI group) or hearts with sham operation (sham group). The dialysate samples were analyzed for adenosine, inosine, hypoxanthine, xanthine, and uric acid. LV volume was greater in the MI group (2.2 ± 0.2 ml/kg) compared with the sham group (1.3 ± 0.2 ml/kg, P < 0.05). Infarct size was 28 ± 4%. The baseline dialysate level of uric acid was higher in the MI group (18.9 ± 3.4 μmol) compared with the sham group (4.6 ± 0.7 μmol, P < 0.01). During and after Iso infusion, the dialysate levels of adenosine, xanthine, and uric acid were all significantly higher in the MI group. Thus the level of IPM is increased in hearts with postinfarction LV remodeling both at baseline and during Iso infusion. These results suggest that the decreased myocardial ATP level in hearts with post-MI LV remodeling may be caused by the chronic depletion of the total adenine nucleotide pool.

Original languageEnglish (US)
Pages (from-to)H677-H684
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume286
Issue number2 55-2
StatePublished - Feb 1 2004
Externally publishedYes

Keywords

  • ATP
  • Heart failure
  • Left ventricle
  • β-adrenergic receptors

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

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  • Cite this

    Gourine, A. V., Hu, Q., Sander, P. R., Kuzmin, A. I., Hanafy, N., Davydova, S. A., Zaretsky, D. V., & Zhang, J. (2004). Interstitial purine metabolites in hearts with LV remodeling. American Journal of Physiology - Heart and Circulatory Physiology, 286(2 55-2), H677-H684.