Intracellular calcium dynamics and acetylcholine-induced triggered activity in the pulmonary veins of dogs with pacing-induced heart failure

Chung Chuan Chou, Bich Lien Nguyen, Alex Y. Tan, Po Cheng Chang, Hui Ling Lee, Fun Chung Lin, San Jou Yeh, Michael C. Fishbein, Shien-Fong Lin, Delon Wu, Ming Shien Wen, Peng-Sheng Chen

Research output: Contribution to journalArticle

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Abstract

Background: Heart failure increases autonomic nerve activities and changes intracellular calcium (Cai) dynamics. Objective: The purpose of this study was to investigate the hypothesis that abnormal Cai dynamics are responsible for triggered activity in the pulmonary veins (PVs) during acetylcholine infusion in a canine model of heart failure. Methods: Simultaneous optical mapping of Cai and membrane potential was performed in isolated Langendorff-perfused PV-left atrial (LA) preparations from nine dogs with ventricular pacing-induced heart failure. Mapping was performed at baseline, during acetylcholine (1 μmol/L) infusion (N = 9), and during thapsigargin and ryanodine infusion (N = 6). Results: Acetylcholine abbreviated the action potential. In four tissues, long pauses were followed by elevated diastolic Cai, late phase 3 early afterdepolarizations, and atrial fibrillation (AF). The incidence of PV focal discharges during AF was increased by acetylcholine from 2.4 ± 0.6 beats/s (N = 4) to 6.5 ± 2.2 beats/s (N = 8; P = .003). PV focal discharge and PV-LA microreentry coexisted in 6 of 9 preparations. The spatial distribution of dominant frequency demonstrated a focal source pattern, with the highest dominant frequency areas colocalized with PV focal discharge sites in 35 (95%) of 37 cholinergic AF episodes (N = 8). Thapsigargin and ryanodine infusion eliminated focal discharges in 6 of 6 preparations and suppressed the inducibility of AF in 4 of 6 preparations. PVs with focal discharge have higher densities of parasympathetic nerves than do PVs without focal discharges (P = .01), and periodic acid-Schiff (PAS)-positive cells were present at the focal discharge sites. Conclusion: Cai dynamics are important in promoting triggered activity during acetylcholine infusion in PVs from pacing-induced heart failure. PV focal discharge sites have PAS-positive cells and high densities of parasympathetic nerves.

Original languageEnglish
Pages (from-to)1170-1177
Number of pages8
JournalHeart Rhythm
Volume5
Issue number8
DOIs
StatePublished - Aug 2008

Fingerprint

Pulmonary Veins
Acetylcholine
Heart Failure
Dogs
Calcium
Atrial Fibrillation
Ryanodine
Periodic Acid
Thapsigargin
Autonomic Pathways
Membrane Potentials
Cholinergic Agents
Action Potentials
Canidae
Cell Count

Keywords

  • Acetylcholine
  • Atrium
  • Calcium
  • Fibrillation
  • Heart failure
  • Mapping

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Intracellular calcium dynamics and acetylcholine-induced triggered activity in the pulmonary veins of dogs with pacing-induced heart failure. / Chou, Chung Chuan; Nguyen, Bich Lien; Tan, Alex Y.; Chang, Po Cheng; Lee, Hui Ling; Lin, Fun Chung; Yeh, San Jou; Fishbein, Michael C.; Lin, Shien-Fong; Wu, Delon; Wen, Ming Shien; Chen, Peng-Sheng.

In: Heart Rhythm, Vol. 5, No. 8, 08.2008, p. 1170-1177.

Research output: Contribution to journalArticle

Chou, Chung Chuan ; Nguyen, Bich Lien ; Tan, Alex Y. ; Chang, Po Cheng ; Lee, Hui Ling ; Lin, Fun Chung ; Yeh, San Jou ; Fishbein, Michael C. ; Lin, Shien-Fong ; Wu, Delon ; Wen, Ming Shien ; Chen, Peng-Sheng. / Intracellular calcium dynamics and acetylcholine-induced triggered activity in the pulmonary veins of dogs with pacing-induced heart failure. In: Heart Rhythm. 2008 ; Vol. 5, No. 8. pp. 1170-1177.
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abstract = "Background: Heart failure increases autonomic nerve activities and changes intracellular calcium (Cai) dynamics. Objective: The purpose of this study was to investigate the hypothesis that abnormal Cai dynamics are responsible for triggered activity in the pulmonary veins (PVs) during acetylcholine infusion in a canine model of heart failure. Methods: Simultaneous optical mapping of Cai and membrane potential was performed in isolated Langendorff-perfused PV-left atrial (LA) preparations from nine dogs with ventricular pacing-induced heart failure. Mapping was performed at baseline, during acetylcholine (1 μmol/L) infusion (N = 9), and during thapsigargin and ryanodine infusion (N = 6). Results: Acetylcholine abbreviated the action potential. In four tissues, long pauses were followed by elevated diastolic Cai, late phase 3 early afterdepolarizations, and atrial fibrillation (AF). The incidence of PV focal discharges during AF was increased by acetylcholine from 2.4 ± 0.6 beats/s (N = 4) to 6.5 ± 2.2 beats/s (N = 8; P = .003). PV focal discharge and PV-LA microreentry coexisted in 6 of 9 preparations. The spatial distribution of dominant frequency demonstrated a focal source pattern, with the highest dominant frequency areas colocalized with PV focal discharge sites in 35 (95{\%}) of 37 cholinergic AF episodes (N = 8). Thapsigargin and ryanodine infusion eliminated focal discharges in 6 of 6 preparations and suppressed the inducibility of AF in 4 of 6 preparations. PVs with focal discharge have higher densities of parasympathetic nerves than do PVs without focal discharges (P = .01), and periodic acid-Schiff (PAS)-positive cells were present at the focal discharge sites. Conclusion: Cai dynamics are important in promoting triggered activity during acetylcholine infusion in PVs from pacing-induced heart failure. PV focal discharge sites have PAS-positive cells and high densities of parasympathetic nerves.",
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T1 - Intracellular calcium dynamics and acetylcholine-induced triggered activity in the pulmonary veins of dogs with pacing-induced heart failure

AU - Chou, Chung Chuan

AU - Nguyen, Bich Lien

AU - Tan, Alex Y.

AU - Chang, Po Cheng

AU - Lee, Hui Ling

AU - Lin, Fun Chung

AU - Yeh, San Jou

AU - Fishbein, Michael C.

AU - Lin, Shien-Fong

AU - Wu, Delon

AU - Wen, Ming Shien

AU - Chen, Peng-Sheng

PY - 2008/8

Y1 - 2008/8

N2 - Background: Heart failure increases autonomic nerve activities and changes intracellular calcium (Cai) dynamics. Objective: The purpose of this study was to investigate the hypothesis that abnormal Cai dynamics are responsible for triggered activity in the pulmonary veins (PVs) during acetylcholine infusion in a canine model of heart failure. Methods: Simultaneous optical mapping of Cai and membrane potential was performed in isolated Langendorff-perfused PV-left atrial (LA) preparations from nine dogs with ventricular pacing-induced heart failure. Mapping was performed at baseline, during acetylcholine (1 μmol/L) infusion (N = 9), and during thapsigargin and ryanodine infusion (N = 6). Results: Acetylcholine abbreviated the action potential. In four tissues, long pauses were followed by elevated diastolic Cai, late phase 3 early afterdepolarizations, and atrial fibrillation (AF). The incidence of PV focal discharges during AF was increased by acetylcholine from 2.4 ± 0.6 beats/s (N = 4) to 6.5 ± 2.2 beats/s (N = 8; P = .003). PV focal discharge and PV-LA microreentry coexisted in 6 of 9 preparations. The spatial distribution of dominant frequency demonstrated a focal source pattern, with the highest dominant frequency areas colocalized with PV focal discharge sites in 35 (95%) of 37 cholinergic AF episodes (N = 8). Thapsigargin and ryanodine infusion eliminated focal discharges in 6 of 6 preparations and suppressed the inducibility of AF in 4 of 6 preparations. PVs with focal discharge have higher densities of parasympathetic nerves than do PVs without focal discharges (P = .01), and periodic acid-Schiff (PAS)-positive cells were present at the focal discharge sites. Conclusion: Cai dynamics are important in promoting triggered activity during acetylcholine infusion in PVs from pacing-induced heart failure. PV focal discharge sites have PAS-positive cells and high densities of parasympathetic nerves.

AB - Background: Heart failure increases autonomic nerve activities and changes intracellular calcium (Cai) dynamics. Objective: The purpose of this study was to investigate the hypothesis that abnormal Cai dynamics are responsible for triggered activity in the pulmonary veins (PVs) during acetylcholine infusion in a canine model of heart failure. Methods: Simultaneous optical mapping of Cai and membrane potential was performed in isolated Langendorff-perfused PV-left atrial (LA) preparations from nine dogs with ventricular pacing-induced heart failure. Mapping was performed at baseline, during acetylcholine (1 μmol/L) infusion (N = 9), and during thapsigargin and ryanodine infusion (N = 6). Results: Acetylcholine abbreviated the action potential. In four tissues, long pauses were followed by elevated diastolic Cai, late phase 3 early afterdepolarizations, and atrial fibrillation (AF). The incidence of PV focal discharges during AF was increased by acetylcholine from 2.4 ± 0.6 beats/s (N = 4) to 6.5 ± 2.2 beats/s (N = 8; P = .003). PV focal discharge and PV-LA microreentry coexisted in 6 of 9 preparations. The spatial distribution of dominant frequency demonstrated a focal source pattern, with the highest dominant frequency areas colocalized with PV focal discharge sites in 35 (95%) of 37 cholinergic AF episodes (N = 8). Thapsigargin and ryanodine infusion eliminated focal discharges in 6 of 6 preparations and suppressed the inducibility of AF in 4 of 6 preparations. PVs with focal discharge have higher densities of parasympathetic nerves than do PVs without focal discharges (P = .01), and periodic acid-Schiff (PAS)-positive cells were present at the focal discharge sites. Conclusion: Cai dynamics are important in promoting triggered activity during acetylcholine infusion in PVs from pacing-induced heart failure. PV focal discharge sites have PAS-positive cells and high densities of parasympathetic nerves.

KW - Acetylcholine

KW - Atrium

KW - Calcium

KW - Fibrillation

KW - Heart failure

KW - Mapping

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