Intracellular fragmentation of bone resorption products by reactive oxygen species generated by osteoclastic tartrate-resistant acid phosphatase

Jussi M. Halleen, Seija Räisänen, Jari J. Salo, Sakamuri V. Reddy, G. David Roodman, Teuvo A. Hentunen, Petri P. Lehenkari, Helena Kaija, Pirkko Vihko, H. Kalervo Väänänen

Research output: Contribution to journalArticle

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Abstract

Tartrate-resistant acid phosphatase (TRAP) is highly expressed in bone- resorbing osteoclasts and activated macrophages. It has been suggested that a redox-active iron in the binuclear iron center of TRAP could have the capacity to react with hydrogen peroxide to produce highly destructive reactive oxygen species (ROS). Here we show that TRAP can generate ROS in vitro and that cells over-expressing TRAP produce higher amounts of intracellular ROS than their parent cells. We further demonstrate that these ROS can be targeted to destroy collagen and other proteins. In resorbing osteoclasts, TRAP was found in transcytotic vesicles transporting matrix degradation products through the cell, suggesting that TRAP-facilitated fragmentation of endocytosed material takes place in a specific cellular compartment. These results suggest that bone matrix degradation occurs not only extracellularly in the resorption lacunae but also intracellularly in the transcytotic vesicles. We propose that proteins containing redox-active iron could represent a novel mechanism of physiological fragmentation of organic molecules. This mechanism could be important in tissue remodeling and as a defense mechanism of phagocytosing cells.

Original languageEnglish (US)
Pages (from-to)22907-22910
Number of pages4
JournalJournal of Biological Chemistry
Volume274
Issue number33
DOIs
StatePublished - Aug 13 1999
Externally publishedYes

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Bone Resorption
Acid Phosphatase
Reactive Oxygen Species
Bone
Iron
Osteoclasts
Oxidation-Reduction
Cytophagocytosis
Degradation
Bone Matrix
Macrophages
Endocytosis
Hydrogen Peroxide
Tartrate-Resistant Acid Phosphatase
tartaric acid
Proteins
Collagen
Tissue
Bone and Bones
Molecules

ASJC Scopus subject areas

  • Biochemistry

Cite this

Intracellular fragmentation of bone resorption products by reactive oxygen species generated by osteoclastic tartrate-resistant acid phosphatase. / Halleen, Jussi M.; Räisänen, Seija; Salo, Jari J.; Reddy, Sakamuri V.; Roodman, G. David; Hentunen, Teuvo A.; Lehenkari, Petri P.; Kaija, Helena; Vihko, Pirkko; Väänänen, H. Kalervo.

In: Journal of Biological Chemistry, Vol. 274, No. 33, 13.08.1999, p. 22907-22910.

Research output: Contribution to journalArticle

Halleen, JM, Räisänen, S, Salo, JJ, Reddy, SV, Roodman, GD, Hentunen, TA, Lehenkari, PP, Kaija, H, Vihko, P & Väänänen, HK 1999, 'Intracellular fragmentation of bone resorption products by reactive oxygen species generated by osteoclastic tartrate-resistant acid phosphatase', Journal of Biological Chemistry, vol. 274, no. 33, pp. 22907-22910. https://doi.org/10.1074/jbc.274.33.22907
Halleen, Jussi M. ; Räisänen, Seija ; Salo, Jari J. ; Reddy, Sakamuri V. ; Roodman, G. David ; Hentunen, Teuvo A. ; Lehenkari, Petri P. ; Kaija, Helena ; Vihko, Pirkko ; Väänänen, H. Kalervo. / Intracellular fragmentation of bone resorption products by reactive oxygen species generated by osteoclastic tartrate-resistant acid phosphatase. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 33. pp. 22907-22910.
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