Intracellular location of rabbit poxvirus nucleic acid within infected cells as determined by in situ hybridization

H. Minnigan, R. W. Moyer

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The intracellular location of rabbit poxvirus DNA within cells during the course of infection has been determined by the hybridization in situ of labeled viral DNA probes to uninfected and infected cells under various conditions. Extensive control experiments were performed to demonstrate that DNA could be detected selectively and accurately within the cell. Our results suggest that rabbit poxvirus DNA is located only within the cytoplasm during the reproductive cycle, and we found no evidence that viral DNA enters the cell nucleus. The pattern of hybridization of viral DNA at early times (1 and 2 h postinfection) and in the presence of inhibitors of viral DNA synthesis suggests that there may be an association between the input viral DNA and some structural component of the host cell. A number of observations support the hypothesis that the host cell nucleus is required for a productive poxvirus infection. Our results are discussed in terms of the possible role of the nucleus in the replication of poxviruses.

Original languageEnglish (US)
Pages (from-to)634-643
Number of pages10
JournalJournal of virology
Volume55
Issue number3
StatePublished - Dec 1 1985

Fingerprint

Poxviridae
Viral DNA
in situ hybridization
Nucleic Acids
nucleic acids
In Situ Hybridization
rabbits
Rabbits
DNA
Cell Nucleus
cells
Poxviridae Infections
cell nucleus
Nucleic Acid Synthesis Inhibitors
DNA Probes
Cellular Structures
Cytoplasm
DNA probes
infection
hybridization

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Intracellular location of rabbit poxvirus nucleic acid within infected cells as determined by in situ hybridization. / Minnigan, H.; Moyer, R. W.

In: Journal of virology, Vol. 55, No. 3, 01.12.1985, p. 634-643.

Research output: Contribution to journalArticle

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