Intracellular Na+ overload causes oxidation of CaMKII and leads to Ca2+ mishandling in isolated ventricular myocytes

Serge Viatchenko-Karpinski, Dmytro Kornyeyev, Nesrine El-Bizri, Grant Budas, Peidong Fan, Zhan Jiang, Jin Yang, Mark E. Anderson, John C. Shryock, Ching-Pin Chang, Luiz Belardinelli, Lina Yao

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

An increase of late Na+ current (INaL) in cardiac myocytes can raise the cytosolic Na+ concentration and is associated with activation of Ca2+/calmodulin-dependent protein kinase II (CaMKII) and alterations of mitochondrial metabolism and Ca2+ handling by sarcoplasmic reticulum (SR). We tested the hypothesis that augmentation of INaL can increase mitochondrial reactive oxygen species (ROS) production and oxidation of CaMKII, resulting in spontaneous SR Ca2+ release and increased diastolic Ca2+ in myocytes. Increases of INaL and/or of the cytosolic Na+ concentration led to mitochondrial ROS production and oxidation of CaMKII to cause dysregulation of Ca2+ handling in rabbit cardiac myocytes.

Original languageEnglish
Pages (from-to)247-256
Number of pages10
JournalJournal of Molecular and Cellular Cardiology
Volume76
DOIs
StatePublished - Nov 1 2014

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Calcium-Calmodulin-Dependent Protein Kinase Type 2
Muscle Cells
Sarcoplasmic Reticulum
Cardiac Myocytes
Reactive Oxygen Species
Rabbits

Keywords

  • ATX-II
  • CaMKII
  • Late sodium current
  • Mitochondria
  • ROS
  • RyRs

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Cite this

Intracellular Na+ overload causes oxidation of CaMKII and leads to Ca2+ mishandling in isolated ventricular myocytes. / Viatchenko-Karpinski, Serge; Kornyeyev, Dmytro; El-Bizri, Nesrine; Budas, Grant; Fan, Peidong; Jiang, Zhan; Yang, Jin; Anderson, Mark E.; Shryock, John C.; Chang, Ching-Pin; Belardinelli, Luiz; Yao, Lina.

In: Journal of Molecular and Cellular Cardiology, Vol. 76, 01.11.2014, p. 247-256.

Research output: Contribution to journalArticle

Viatchenko-Karpinski, S, Kornyeyev, D, El-Bizri, N, Budas, G, Fan, P, Jiang, Z, Yang, J, Anderson, ME, Shryock, JC, Chang, C-P, Belardinelli, L & Yao, L 2014, 'Intracellular Na+ overload causes oxidation of CaMKII and leads to Ca2+ mishandling in isolated ventricular myocytes', Journal of Molecular and Cellular Cardiology, vol. 76, pp. 247-256. https://doi.org/10.1016/j.yjmcc.2014.09.009
Viatchenko-Karpinski, Serge ; Kornyeyev, Dmytro ; El-Bizri, Nesrine ; Budas, Grant ; Fan, Peidong ; Jiang, Zhan ; Yang, Jin ; Anderson, Mark E. ; Shryock, John C. ; Chang, Ching-Pin ; Belardinelli, Luiz ; Yao, Lina. / Intracellular Na+ overload causes oxidation of CaMKII and leads to Ca2+ mishandling in isolated ventricular myocytes. In: Journal of Molecular and Cellular Cardiology. 2014 ; Vol. 76. pp. 247-256.
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