Intracellular Na+ overload causes oxidation of CaMKII and leads to Ca2+ mishandling in isolated ventricular myocytes

Serge Viatchenko-Karpinski, Dmytro Kornyeyev, Nesrine El-Bizri, Grant Budas, Peidong Fan, Zhan Jiang, Jin Yang, Mark E. Anderson, John C. Shryock, Ching Pin Chang, Luiz Belardinelli, Lina Yao

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

An increase of late Na+ current (INaL) in cardiac myocytes can raise the cytosolic Na+ concentration and is associated with activation of Ca2+/calmodulin-dependent protein kinase II (CaMKII) and alterations of mitochondrial metabolism and Ca2+ handling by sarcoplasmic reticulum (SR). We tested the hypothesis that augmentation of INaL can increase mitochondrial reactive oxygen species (ROS) production and oxidation of CaMKII, resulting in spontaneous SR Ca2+ release and increased diastolic Ca2+ in myocytes. Increases of INaL and/or of the cytosolic Na+ concentration led to mitochondrial ROS production and oxidation of CaMKII to cause dysregulation of Ca2+ handling in rabbit cardiac myocytes.

Original languageEnglish (US)
Pages (from-to)247-256
Number of pages10
JournalJournal of Molecular and Cellular Cardiology
Volume76
DOIs
StatePublished - Nov 1 2014

Keywords

  • ATX-II
  • CaMKII
  • Late sodium current
  • Mitochondria
  • ROS
  • RyRs

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

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    Viatchenko-Karpinski, S., Kornyeyev, D., El-Bizri, N., Budas, G., Fan, P., Jiang, Z., Yang, J., Anderson, M. E., Shryock, J. C., Chang, C. P., Belardinelli, L., & Yao, L. (2014). Intracellular Na+ overload causes oxidation of CaMKII and leads to Ca2+ mishandling in isolated ventricular myocytes. Journal of Molecular and Cellular Cardiology, 76, 247-256. https://doi.org/10.1016/j.yjmcc.2014.09.009