Intracoronary administration of streptokinase in a canine model disturbances in thromboxane a2-prostacyclin balance

Gary J. Becker, Donald L. Kreipke, Robert W. Holden, R. Gerald Dreesen, Kenneth Ryder, Bruce Petersen, Andrew P. Evan, Phillip J. Bendick, Thomas D. Franklin, Eugene C. Klatte

Research output: Contribution to journalArticle

Abstract

The potential impact of local intracoronary infusion of streptokinase (SK) on vascular prostaglandin synthetic pathways was studied in a canine model. Control animals (n = 10) underwent left coronary artery (LC A) infusion of50,000 units SK for 90 minutes; experimental animals (n = 10) underwent LC A infusion of normal saline. Plasma samples for radioimmunoassay (RI A) of prostacyclin (PGI-2) and thromboxane (TXA-2) were obtained from the coronary sinus (CS) as follows: One sample pre infusion, six samples during infusion, and three samples post in fusion in each animal. Comparisons between control and experimental plasma levels of PGI-2 and TXA-2 were made for each sampling time. The PGI-2 levels remained at or below the lower limits of detect ability by RIA (the most sensitive assay available) in both control and experimental animals. TXA-2 levels were higher in experimental than in control animals at all sampling times, with the most significant differences occurring in samples 3 (after 30 minutes of infusion,.001 <P <.01), 4 (after 45 minutes of infusion,.05 <P <.10), and 5 (after 60 minutes of infusion,.02 < P <.05). We suggest (1) it is unlikely that any of the beneficial effects of coronary streptokinase infusions are PGI-2-mediated, (2) that the TXA-2 increases in our model may represent a patho physiologic-biochemical correlate of previously identified morphologic evidence of endothelial damage in animals infused with fibrinolytic agents, and (3) that our findings may indicate that fibrinolytic infusions produce competing effects: Lysis of thrombi and endothelial injury with TXA-2 production. Investigation into a possible dose response relationship and possible adjunctive use of antiplatelet drugs, antithromboxane drugs, and calcium channel blockers is warranted.

Original languageEnglish (US)
Pages (from-to)206-209
Number of pages4
JournalInvestigative Radiology
Volume21
Issue number3
DOIs
StatePublished - Mar 1986

Keywords

  • Animal studies
  • Coronary arteries
  • Fibrinolysis
  • Prostaglandins

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

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    Becker, G. J., Kreipke, D. L., Holden, R. W., Gerald Dreesen, R., Ryder, K., Petersen, B., Evan, A. P., Bendick, P. J., Franklin, T. D., & Klatte, E. C. (1986). Intracoronary administration of streptokinase in a canine model disturbances in thromboxane a2-prostacyclin balance. Investigative Radiology, 21(3), 206-209. https://doi.org/10.1097/00004424-198603000-00003