Intramyocardial transplantation of human adipose-derived stromal cell and endothelial progenitor cell mixture was not superior to individual cell type transplantation in improving left ventricular function in rats with myocardial infarction

Soon Jun Hong, John Kihlken, Seung Cheol Choi, Keith L. March, Do Sun Lim

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15 Citations (Scopus)

Abstract

Background: Both adipose-derived stromal cells (ASCs) and endothelial progenitor cells (EPCs) have high potential for promoting tissue revascularization and functional recovery in acute myocardial infarction (AMI) models. We investigated the functional effects of intramyocardial transplantation of a human ASC and EPC mixture in immunodeficient rats after MI. Methods: MI was induced by ligating left anterior descending coronary artery. Survived rats were randomly assigned to 1 of 4 different groups: the control group (n = 10, saline in 100 μL), the ASC group (n = 10, 106 ASCs), the EPC group (n = 10, 106 EPCs), or the ASC + EPC group (n = 10, 2 × 105 ASCs + 8 × 105 EPCs). Left ventricular (LV) function was compared using echocardiography during the 28-day follow-up. GAP43+ nerve sprouting and smooth muscle α-actin + angiogenesis were also compared. Results: Serial changes in LV ejection fraction (EF) and fractional shortening revealed significant increases in the ASC, EPC, and ASC + EPC groups when compared to the control group during the follow-up (49 ± 3%, 49 ± 4%, 47 ± 4%, 39 ± 2%, P < 0.001, respectively for LVEF) (33 ± 4%, 32 ± 2%, 31 ± 2%, 23 ± 2%, P = 0.002, respectively for fractional shortening). The number of α-actin + arterioles and GAP43+ nerve area was significantly greater in the ASC, EPC, and ASC + EPC groups when compared to the control group in the peri-infarct area (34.4 ± 1.0/mm2, 35.9 ± 1.1/mm2, 35.3 ± 0.9/mm2, 17.4 ± 0.7/mm2, P < 0.001, respectively for angiogenesis) (346.2 ± 10.7 μm2/mm 2, 357.2 ± 12.8 μm2/mm2, 368.0 ± 9.7 μm2/mm2, 174.6 ± 7.9 μm 2/mm2, P < 0.001, respectively for nerve sprouting). Conlusions: Intramyocardial injections of ASCs, EPCs, or ASCs + EPCs are effective modalities for the treatment of myocardial damage in rats and may expand the potential clinical application of ASC or EPC therapy in patients with ischemic heart disease.

Original languageEnglish
Pages (from-to)205-211
Number of pages7
JournalInternational Journal of Cardiology
Volume164
Issue number2
DOIs
StatePublished - Apr 5 2013

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Cell Transplantation
Stromal Cells
Left Ventricular Function
Transplantation
Myocardial Infarction
Endothelial Progenitor Cells
Control Groups
Actins
Arterioles
Cell- and Tissue-Based Therapy
Stroke Volume
Myocardial Ischemia
Smooth Muscle
Echocardiography
Coronary Vessels

Keywords

  • Adipose
  • Angiogenesis
  • Endothelial progenitor cell
  • Myocardial infarction
  • Stem cell

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

@article{49293f76afc4471c834b812dce7ff471,
title = "Intramyocardial transplantation of human adipose-derived stromal cell and endothelial progenitor cell mixture was not superior to individual cell type transplantation in improving left ventricular function in rats with myocardial infarction",
abstract = "Background: Both adipose-derived stromal cells (ASCs) and endothelial progenitor cells (EPCs) have high potential for promoting tissue revascularization and functional recovery in acute myocardial infarction (AMI) models. We investigated the functional effects of intramyocardial transplantation of a human ASC and EPC mixture in immunodeficient rats after MI. Methods: MI was induced by ligating left anterior descending coronary artery. Survived rats were randomly assigned to 1 of 4 different groups: the control group (n = 10, saline in 100 μL), the ASC group (n = 10, 106 ASCs), the EPC group (n = 10, 106 EPCs), or the ASC + EPC group (n = 10, 2 × 105 ASCs + 8 × 105 EPCs). Left ventricular (LV) function was compared using echocardiography during the 28-day follow-up. GAP43+ nerve sprouting and smooth muscle α-actin + angiogenesis were also compared. Results: Serial changes in LV ejection fraction (EF) and fractional shortening revealed significant increases in the ASC, EPC, and ASC + EPC groups when compared to the control group during the follow-up (49 ± 3{\%}, 49 ± 4{\%}, 47 ± 4{\%}, 39 ± 2{\%}, P < 0.001, respectively for LVEF) (33 ± 4{\%}, 32 ± 2{\%}, 31 ± 2{\%}, 23 ± 2{\%}, P = 0.002, respectively for fractional shortening). The number of α-actin + arterioles and GAP43+ nerve area was significantly greater in the ASC, EPC, and ASC + EPC groups when compared to the control group in the peri-infarct area (34.4 ± 1.0/mm2, 35.9 ± 1.1/mm2, 35.3 ± 0.9/mm2, 17.4 ± 0.7/mm2, P < 0.001, respectively for angiogenesis) (346.2 ± 10.7 μm2/mm 2, 357.2 ± 12.8 μm2/mm2, 368.0 ± 9.7 μm2/mm2, 174.6 ± 7.9 μm 2/mm2, P < 0.001, respectively for nerve sprouting). Conlusions: Intramyocardial injections of ASCs, EPCs, or ASCs + EPCs are effective modalities for the treatment of myocardial damage in rats and may expand the potential clinical application of ASC or EPC therapy in patients with ischemic heart disease.",
keywords = "Adipose, Angiogenesis, Endothelial progenitor cell, Myocardial infarction, Stem cell",
author = "Hong, {Soon Jun} and John Kihlken and Choi, {Seung Cheol} and March, {Keith L.} and Lim, {Do Sun}",
year = "2013",
month = "4",
day = "5",
doi = "10.1016/j.ijcard.2011.06.128",
language = "English",
volume = "164",
pages = "205--211",
journal = "International Journal of Cardiology",
issn = "0167-5273",
publisher = "Elsevier Ireland Ltd",
number = "2",

}

TY - JOUR

T1 - Intramyocardial transplantation of human adipose-derived stromal cell and endothelial progenitor cell mixture was not superior to individual cell type transplantation in improving left ventricular function in rats with myocardial infarction

AU - Hong, Soon Jun

AU - Kihlken, John

AU - Choi, Seung Cheol

AU - March, Keith L.

AU - Lim, Do Sun

PY - 2013/4/5

Y1 - 2013/4/5

N2 - Background: Both adipose-derived stromal cells (ASCs) and endothelial progenitor cells (EPCs) have high potential for promoting tissue revascularization and functional recovery in acute myocardial infarction (AMI) models. We investigated the functional effects of intramyocardial transplantation of a human ASC and EPC mixture in immunodeficient rats after MI. Methods: MI was induced by ligating left anterior descending coronary artery. Survived rats were randomly assigned to 1 of 4 different groups: the control group (n = 10, saline in 100 μL), the ASC group (n = 10, 106 ASCs), the EPC group (n = 10, 106 EPCs), or the ASC + EPC group (n = 10, 2 × 105 ASCs + 8 × 105 EPCs). Left ventricular (LV) function was compared using echocardiography during the 28-day follow-up. GAP43+ nerve sprouting and smooth muscle α-actin + angiogenesis were also compared. Results: Serial changes in LV ejection fraction (EF) and fractional shortening revealed significant increases in the ASC, EPC, and ASC + EPC groups when compared to the control group during the follow-up (49 ± 3%, 49 ± 4%, 47 ± 4%, 39 ± 2%, P < 0.001, respectively for LVEF) (33 ± 4%, 32 ± 2%, 31 ± 2%, 23 ± 2%, P = 0.002, respectively for fractional shortening). The number of α-actin + arterioles and GAP43+ nerve area was significantly greater in the ASC, EPC, and ASC + EPC groups when compared to the control group in the peri-infarct area (34.4 ± 1.0/mm2, 35.9 ± 1.1/mm2, 35.3 ± 0.9/mm2, 17.4 ± 0.7/mm2, P < 0.001, respectively for angiogenesis) (346.2 ± 10.7 μm2/mm 2, 357.2 ± 12.8 μm2/mm2, 368.0 ± 9.7 μm2/mm2, 174.6 ± 7.9 μm 2/mm2, P < 0.001, respectively for nerve sprouting). Conlusions: Intramyocardial injections of ASCs, EPCs, or ASCs + EPCs are effective modalities for the treatment of myocardial damage in rats and may expand the potential clinical application of ASC or EPC therapy in patients with ischemic heart disease.

AB - Background: Both adipose-derived stromal cells (ASCs) and endothelial progenitor cells (EPCs) have high potential for promoting tissue revascularization and functional recovery in acute myocardial infarction (AMI) models. We investigated the functional effects of intramyocardial transplantation of a human ASC and EPC mixture in immunodeficient rats after MI. Methods: MI was induced by ligating left anterior descending coronary artery. Survived rats were randomly assigned to 1 of 4 different groups: the control group (n = 10, saline in 100 μL), the ASC group (n = 10, 106 ASCs), the EPC group (n = 10, 106 EPCs), or the ASC + EPC group (n = 10, 2 × 105 ASCs + 8 × 105 EPCs). Left ventricular (LV) function was compared using echocardiography during the 28-day follow-up. GAP43+ nerve sprouting and smooth muscle α-actin + angiogenesis were also compared. Results: Serial changes in LV ejection fraction (EF) and fractional shortening revealed significant increases in the ASC, EPC, and ASC + EPC groups when compared to the control group during the follow-up (49 ± 3%, 49 ± 4%, 47 ± 4%, 39 ± 2%, P < 0.001, respectively for LVEF) (33 ± 4%, 32 ± 2%, 31 ± 2%, 23 ± 2%, P = 0.002, respectively for fractional shortening). The number of α-actin + arterioles and GAP43+ nerve area was significantly greater in the ASC, EPC, and ASC + EPC groups when compared to the control group in the peri-infarct area (34.4 ± 1.0/mm2, 35.9 ± 1.1/mm2, 35.3 ± 0.9/mm2, 17.4 ± 0.7/mm2, P < 0.001, respectively for angiogenesis) (346.2 ± 10.7 μm2/mm 2, 357.2 ± 12.8 μm2/mm2, 368.0 ± 9.7 μm2/mm2, 174.6 ± 7.9 μm 2/mm2, P < 0.001, respectively for nerve sprouting). Conlusions: Intramyocardial injections of ASCs, EPCs, or ASCs + EPCs are effective modalities for the treatment of myocardial damage in rats and may expand the potential clinical application of ASC or EPC therapy in patients with ischemic heart disease.

KW - Adipose

KW - Angiogenesis

KW - Endothelial progenitor cell

KW - Myocardial infarction

KW - Stem cell

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JO - International Journal of Cardiology

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