Intraperitoneal immunity and pneumoperitoneum

E. G. Chekan, C. Nataraj, E. M. Clary, Thomas Hayward, F. J. Brody, J. C. Stamat, M. C. Fina, W. S. Eubanks, C. J. Westcott

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Background: Carbon dioxide (CO2) pneumoperitoneum has been implicated as a possible factor in depressed intraperitoneal immunity. Using in vitro functional assays, CO2 has been shown to decrease the function of peritoneal macrophages harvested from insufflated mice. However, an effective in vivo assessment is lacking. Listeria monocytogenes (LM), an intracellular pathogen, has served as a well-established in vivo model to study cell- mediated immune responses in mice. This study examines the immune competence of mice based on their ability to clear intraperitoneally administered LM following CO2 vs helium (He) insufflation. Methods: Eighty-five mice (C57B1/6, males, 4-6 weeks old) were divided between the following four treatment groups: CO2 insufflation, He insufflation, abdominal laparotomy (Lap), and control (anesthesia only). Immediately postoperatively, each group was inoculated percutaneously and intraperitoneally with a sublethal dose (.015 x 106 org) of virulent LM (EGD strain). Half of the animals were killed on postoperative day 3 and half on day 5. Spleens and livers (sites of bacterial predilection) were harvested, homogenized, and plated on TSB agar. The amount of bacteria (1 x 106 LM/spleen and liver) from each group was then compared. Statistical significance was set at p ≤ 0.05. Results: Control animals had nominal bacteria on day 3 (0.016 x 106 LM/spleen and liver), and the bacterial burden remained low at day 5 (0.038 x 106 LM/spleen and liver) postchallenge. On day 3, the bacterial burden was significantly higher in the CO2 group (5.46 x 106 LM/spleen and liver) as compared to He (0.093 x 106 LM/spleen and liver) and controls. The Lap group (3.44 x 106 LM/spleen and liver) had significantly more bacteria than the controls. There were no significant differences between any of the groups on day 5. Conclusions: In this animal model, CO2 pneumoperitoneum impaired cell-mediated intraperitoneal immunity significantly more than He pneumoperitoneum and controls on day 3. Also on day 3, laparotomy caused impairment of intraperitoneal immunity when compared to controls. Finally, intraperitoneal immunosuppression resolved by day 5.

Original languageEnglish (US)
Pages (from-to)1135-1138
Number of pages4
JournalSurgical Endoscopy and Other Interventional Techniques
Volume13
Issue number11
DOIs
StatePublished - Nov 1999
Externally publishedYes

Fingerprint

Pneumoperitoneum
Listeria monocytogenes
Immunity
Spleen
Helium
Liver
Insufflation
Laparotomy
Bacteria
Aptitude
Peritoneal Macrophages
Carbon Dioxide
Cellular Immunity
Mental Competency
Immunosuppression
Agar
Anesthesia
Animal Models

Keywords

  • Helium
  • Intraperitoneal immunity
  • Laparoscopy
  • Listeria monocytogenes
  • Pneumoperitoneum

ASJC Scopus subject areas

  • Surgery

Cite this

Chekan, E. G., Nataraj, C., Clary, E. M., Hayward, T., Brody, F. J., Stamat, J. C., ... Westcott, C. J. (1999). Intraperitoneal immunity and pneumoperitoneum. Surgical Endoscopy and Other Interventional Techniques, 13(11), 1135-1138. https://doi.org/10.1007/s004649901189

Intraperitoneal immunity and pneumoperitoneum. / Chekan, E. G.; Nataraj, C.; Clary, E. M.; Hayward, Thomas; Brody, F. J.; Stamat, J. C.; Fina, M. C.; Eubanks, W. S.; Westcott, C. J.

In: Surgical Endoscopy and Other Interventional Techniques, Vol. 13, No. 11, 11.1999, p. 1135-1138.

Research output: Contribution to journalArticle

Chekan, EG, Nataraj, C, Clary, EM, Hayward, T, Brody, FJ, Stamat, JC, Fina, MC, Eubanks, WS & Westcott, CJ 1999, 'Intraperitoneal immunity and pneumoperitoneum', Surgical Endoscopy and Other Interventional Techniques, vol. 13, no. 11, pp. 1135-1138. https://doi.org/10.1007/s004649901189
Chekan, E. G. ; Nataraj, C. ; Clary, E. M. ; Hayward, Thomas ; Brody, F. J. ; Stamat, J. C. ; Fina, M. C. ; Eubanks, W. S. ; Westcott, C. J. / Intraperitoneal immunity and pneumoperitoneum. In: Surgical Endoscopy and Other Interventional Techniques. 1999 ; Vol. 13, No. 11. pp. 1135-1138.
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abstract = "Background: Carbon dioxide (CO2) pneumoperitoneum has been implicated as a possible factor in depressed intraperitoneal immunity. Using in vitro functional assays, CO2 has been shown to decrease the function of peritoneal macrophages harvested from insufflated mice. However, an effective in vivo assessment is lacking. Listeria monocytogenes (LM), an intracellular pathogen, has served as a well-established in vivo model to study cell- mediated immune responses in mice. This study examines the immune competence of mice based on their ability to clear intraperitoneally administered LM following CO2 vs helium (He) insufflation. Methods: Eighty-five mice (C57B1/6, males, 4-6 weeks old) were divided between the following four treatment groups: CO2 insufflation, He insufflation, abdominal laparotomy (Lap), and control (anesthesia only). Immediately postoperatively, each group was inoculated percutaneously and intraperitoneally with a sublethal dose (.015 x 106 org) of virulent LM (EGD strain). Half of the animals were killed on postoperative day 3 and half on day 5. Spleens and livers (sites of bacterial predilection) were harvested, homogenized, and plated on TSB agar. The amount of bacteria (1 x 106 LM/spleen and liver) from each group was then compared. Statistical significance was set at p ≤ 0.05. Results: Control animals had nominal bacteria on day 3 (0.016 x 106 LM/spleen and liver), and the bacterial burden remained low at day 5 (0.038 x 106 LM/spleen and liver) postchallenge. On day 3, the bacterial burden was significantly higher in the CO2 group (5.46 x 106 LM/spleen and liver) as compared to He (0.093 x 106 LM/spleen and liver) and controls. The Lap group (3.44 x 106 LM/spleen and liver) had significantly more bacteria than the controls. There were no significant differences between any of the groups on day 5. Conclusions: In this animal model, CO2 pneumoperitoneum impaired cell-mediated intraperitoneal immunity significantly more than He pneumoperitoneum and controls on day 3. Also on day 3, laparotomy caused impairment of intraperitoneal immunity when compared to controls. Finally, intraperitoneal immunosuppression resolved by day 5.",
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AU - Nataraj, C.

AU - Clary, E. M.

AU - Hayward, Thomas

AU - Brody, F. J.

AU - Stamat, J. C.

AU - Fina, M. C.

AU - Eubanks, W. S.

AU - Westcott, C. J.

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N2 - Background: Carbon dioxide (CO2) pneumoperitoneum has been implicated as a possible factor in depressed intraperitoneal immunity. Using in vitro functional assays, CO2 has been shown to decrease the function of peritoneal macrophages harvested from insufflated mice. However, an effective in vivo assessment is lacking. Listeria monocytogenes (LM), an intracellular pathogen, has served as a well-established in vivo model to study cell- mediated immune responses in mice. This study examines the immune competence of mice based on their ability to clear intraperitoneally administered LM following CO2 vs helium (He) insufflation. Methods: Eighty-five mice (C57B1/6, males, 4-6 weeks old) were divided between the following four treatment groups: CO2 insufflation, He insufflation, abdominal laparotomy (Lap), and control (anesthesia only). Immediately postoperatively, each group was inoculated percutaneously and intraperitoneally with a sublethal dose (.015 x 106 org) of virulent LM (EGD strain). Half of the animals were killed on postoperative day 3 and half on day 5. Spleens and livers (sites of bacterial predilection) were harvested, homogenized, and plated on TSB agar. The amount of bacteria (1 x 106 LM/spleen and liver) from each group was then compared. Statistical significance was set at p ≤ 0.05. Results: Control animals had nominal bacteria on day 3 (0.016 x 106 LM/spleen and liver), and the bacterial burden remained low at day 5 (0.038 x 106 LM/spleen and liver) postchallenge. On day 3, the bacterial burden was significantly higher in the CO2 group (5.46 x 106 LM/spleen and liver) as compared to He (0.093 x 106 LM/spleen and liver) and controls. The Lap group (3.44 x 106 LM/spleen and liver) had significantly more bacteria than the controls. There were no significant differences between any of the groups on day 5. Conclusions: In this animal model, CO2 pneumoperitoneum impaired cell-mediated intraperitoneal immunity significantly more than He pneumoperitoneum and controls on day 3. Also on day 3, laparotomy caused impairment of intraperitoneal immunity when compared to controls. Finally, intraperitoneal immunosuppression resolved by day 5.

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KW - Helium

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KW - Laparoscopy

KW - Listeria monocytogenes

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