Intrastriatal implants of mesencephalic cell suspensions in weaver mutant mice

ultrastructural relationships of dopaminergic dendrites and axons issued from the graft

L. C. Triarhou, P. Brundin, G. Doucet, J. Norton, A. Björklund, Bernardino Ghetti

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Dissociated cell suspensions were prepared from the ventral midbrain of normal mouse foetuses and stereotaxically implanted into the neostriatum of 2-3 months old homozygous weaver mutant mice, which are severely deficient in dopamine. In tests of amphetamine-induced turning behaviour 60 days after grafting, recipient animals displayed a rotational bias opposite to the grafted side. Prior to perfusion, which was carried out at 80 days after transplantation surgery, the grafted striata of the weaver recipients were deprived of their intrinsic mesostriatal dopamine input by local injections of 6-hydroxydopamine into the ipsilateral substantia nigra in order to selectively study the innervation derived from the graft. Grafts were found to contain an estimated 100-700 tyrosine hydroxylase immunoreactive neurones. An ultrastructural analysis demonstrated that both axons and dendrites immunoreactive for tyrosine hydroxylase extended from the graft into the recipient striatum. In the host striatum proximal to the graft (i.e. at a distance of 0.0-0.5 mm from the graft) the proportion of dendrites to axons was about 1:2, whereas distal to the graft (i.e. at a distance of 0.5-1.0 mm) it was 1:20. Graft-derived tyrosine hydroxylase immunoreactive axons were primarily found in apposition with unlabelled dendrites or spines of the recipient striatum (>90%). Graftderived dopaminergic dendrites received synaptic input from unlabelled axon terminals and were opposed to the unlabelled somata of striatal neurones in a few instances. In conclusion, this study shows that mesencephalic cell suspensions survive in the weaver striatum and provide a functional dopamine innervation which comprises both axonal and dendritic processes.

Original languageEnglish
Pages (from-to)3-17
Number of pages15
JournalExperimental Brain Research
Volume79
Issue number1
DOIs
StatePublished - Jan 1990

Fingerprint

Neurologic Mutant Mice
Dendrites
Axons
Suspensions
Transplants
Tyrosine 3-Monooxygenase
Dopamine
Neostriatum
Neurons
Corpus Striatum
Oxidopamine
Presynaptic Terminals
Carisoprodol
Substantia Nigra
Amphetamine
Mesencephalon
Spine
Fetus
Perfusion
Transplantation

Keywords

  • Cell suspension
  • Dopamine
  • Neural graft
  • Substantia nigra
  • Weaver mutant mouse

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Intrastriatal implants of mesencephalic cell suspensions in weaver mutant mice : ultrastructural relationships of dopaminergic dendrites and axons issued from the graft. / Triarhou, L. C.; Brundin, P.; Doucet, G.; Norton, J.; Björklund, A.; Ghetti, Bernardino.

In: Experimental Brain Research, Vol. 79, No. 1, 01.1990, p. 3-17.

Research output: Contribution to journalArticle

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abstract = "Dissociated cell suspensions were prepared from the ventral midbrain of normal mouse foetuses and stereotaxically implanted into the neostriatum of 2-3 months old homozygous weaver mutant mice, which are severely deficient in dopamine. In tests of amphetamine-induced turning behaviour 60 days after grafting, recipient animals displayed a rotational bias opposite to the grafted side. Prior to perfusion, which was carried out at 80 days after transplantation surgery, the grafted striata of the weaver recipients were deprived of their intrinsic mesostriatal dopamine input by local injections of 6-hydroxydopamine into the ipsilateral substantia nigra in order to selectively study the innervation derived from the graft. Grafts were found to contain an estimated 100-700 tyrosine hydroxylase immunoreactive neurones. An ultrastructural analysis demonstrated that both axons and dendrites immunoreactive for tyrosine hydroxylase extended from the graft into the recipient striatum. In the host striatum proximal to the graft (i.e. at a distance of 0.0-0.5 mm from the graft) the proportion of dendrites to axons was about 1:2, whereas distal to the graft (i.e. at a distance of 0.5-1.0 mm) it was 1:20. Graft-derived tyrosine hydroxylase immunoreactive axons were primarily found in apposition with unlabelled dendrites or spines of the recipient striatum (>90{\%}). Graftderived dopaminergic dendrites received synaptic input from unlabelled axon terminals and were opposed to the unlabelled somata of striatal neurones in a few instances. In conclusion, this study shows that mesencephalic cell suspensions survive in the weaver striatum and provide a functional dopamine innervation which comprises both axonal and dendritic processes.",
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