Intravenous cell therapy for acute renal failure with serum amyloid a protein-reprogrammed cells

Katherine Kelly, Barbara Kluve-Beckerman, Jizhong Zhang, Jesus Dominguez

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Serum amyloid A protein (SAA), a prominent component of the acute-phase response, is strongly expressed in developing and repairing kidneys and promotes tubulogenesis. Accordingly, we reprogrammed relatively undifferentiated NRK52E cells with the mouse SAA1.1 gene and transplanted SAA-positive and -negative cells into rats with acute renal failure. We found that SAA-positive cells accelerated renal recovery in three models of acute renal failure: gentamicin nephrotoxicity, cisplatin-mediated renal injury, and ischemia-reperfusion renal injury. The dramatic improvement of renal failure was demonstrable within 2 days, consistent with an early paracrine effect. However, abundant donor cells were also found integrated in the healing tubular architecture after 7 days. We conclude that infusions of SAA-positive cells promote renal recovery after acute renal failure and offer a potentially powerful and novel therapy of renal failure.

Original languageEnglish
JournalAmerican Journal of Physiology - Renal Physiology
Volume299
Issue number2
DOIs
StatePublished - Aug 2010

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Amyloidogenic Proteins
Serum Amyloid A Protein
Cell- and Tissue-Based Therapy
Acute Kidney Injury
Blood Proteins
Kidney
Renal Insufficiency
Acute-Phase Reaction
Gentamicins
Reperfusion Injury
Cisplatin
Ischemia
Wounds and Injuries
Genes

Keywords

  • Acute-phase response
  • Tubulogenesis

ASJC Scopus subject areas

  • Physiology
  • Urology

Cite this

Intravenous cell therapy for acute renal failure with serum amyloid a protein-reprogrammed cells. / Kelly, Katherine; Kluve-Beckerman, Barbara; Zhang, Jizhong; Dominguez, Jesus.

In: American Journal of Physiology - Renal Physiology, Vol. 299, No. 2, 08.2010.

Research output: Contribution to journalArticle

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