Intravital imaging of the kidney

Takashi Hato, Seth Winfree, Pierre Dagher

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Two-photon intravital microscopy is a powerful tool that allows the examination of dynamic cellular processes in the live animal with unprecedented resolution. Indeed, it offers the ability to address unique biological questions that may not be solved by other means. While two-photon intravital microscopy has been successfully applied to study many organs, the kidney presents its own unique challenges that need to be overcome in order to optimize and validate imaging data. For kidney imaging, the complexity of renal architecture and salient autofluorescence merit special considerations as these elements directly impact image acquisition and data interpretation. Here, using illustrative cases, we provide practical guides and discuss issues that may arise during two-photon live imaging of the rodent kidney.

Original languageEnglish (US)
JournalMethods
DOIs
StateAccepted/In press - Jan 23 2017

Fingerprint

Photons
Kidney
Imaging techniques
Image acquisition
Animals
Rodentia
Intravital Microscopy

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Intravital imaging of the kidney. / Hato, Takashi; Winfree, Seth; Dagher, Pierre.

In: Methods, 23.01.2017.

Research output: Contribution to journalArticle

Hato, Takashi ; Winfree, Seth ; Dagher, Pierre. / Intravital imaging of the kidney. In: Methods. 2017.
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