Real time high quality imaging of intra-thoracic organs with sufficient resolution to study microcirculatory dynamics, inflammatory cell trafficking, and cellular and subcellular events during physiologic circulatory and breathing conditions remains a high priority in pulmonary and cardiovascular research. Recent technological developments especially in the area of two-photonmicroscopy (TPM) offer enhanced resolution and dEeper penetration under the organ surface, thus allowing sampling of areas of interest to biologists and physiologists. Furthermore, with TPM one can image sub-cellular and molecular events in real time, such as protein trafficking, enzyme activation, and reactive oxygen species generation, which are pertinent to the pathogenesis of many diseases of interest to the research community. The application of TPM to organs in the thoracic cavity, and especially the lung has been hampered by cardiorespiratory motion and new techniques to mitigate these limitations have been developed. In this chapter, we will describe intravital imaging techniques applied to the lung through a historical perspective, and highlight several recent practical applications of these approaches.
|Original language||English (US)|
|Title of host publication||Advances in Intravital Microscopy|
|Subtitle of host publication||From Basic to Clinical Research|
|Number of pages||12|
|State||Published - Jan 1 2014|
- Real time
ASJC Scopus subject areas