Intravital multiphoton microscopy as a tool for studying renal physiology and pathophysiology

Ruben M. Sandoval, Bruce Molitoris

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The kidney is a complex and dynamic organ with over 40 cell types, and tremendous structural and functional diversity. Intravital multi-photon microscopy, development of fluorescent probes and innovative software, have rapidly advanced the study of intracellular and intercellular processes within the kidney. Researchers can quantify the distribution, behavior, and dynamic interactions of up to four labeled chemical probes and proteins simultaneously and repeatedly in four dimensions (time), with subcellular resolution in near real time. Thus, multi-photon microscopy has greatly extended our ability to investigate cell biology intravitally, at cellular and subcellular resolutions. Therefore, the purpose of the chapter is to demonstrate how the use in intravital multi-photon microscopy has advanced the understanding of both the physiology and pathophysiology of the kidney.

Original languageEnglish (US)
JournalMethods
DOIs
StateAccepted/In press - 2017

Fingerprint

Physiology
Photons
Microscopy
Microscopic examination
Kidney
Cytology
Fluorescent Dyes
Cell Biology
Software
Research Personnel
Intravital Microscopy
Proteins

Keywords

  • Endocytosis
  • Glomerular filtration
  • Kidney
  • Microvasculature
  • Structure-function relationship

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Intravital multiphoton microscopy as a tool for studying renal physiology and pathophysiology. / Sandoval, Ruben M.; Molitoris, Bruce.

In: Methods, 2017.

Research output: Contribution to journalArticle

@article{58f4ff26a3af4a01a42ddea180064333,
title = "Intravital multiphoton microscopy as a tool for studying renal physiology and pathophysiology",
abstract = "The kidney is a complex and dynamic organ with over 40 cell types, and tremendous structural and functional diversity. Intravital multi-photon microscopy, development of fluorescent probes and innovative software, have rapidly advanced the study of intracellular and intercellular processes within the kidney. Researchers can quantify the distribution, behavior, and dynamic interactions of up to four labeled chemical probes and proteins simultaneously and repeatedly in four dimensions (time), with subcellular resolution in near real time. Thus, multi-photon microscopy has greatly extended our ability to investigate cell biology intravitally, at cellular and subcellular resolutions. Therefore, the purpose of the chapter is to demonstrate how the use in intravital multi-photon microscopy has advanced the understanding of both the physiology and pathophysiology of the kidney.",
keywords = "Endocytosis, Glomerular filtration, Kidney, Microvasculature, Structure-function relationship",
author = "Sandoval, {Ruben M.} and Bruce Molitoris",
year = "2017",
doi = "10.1016/j.ymeth.2017.07.014",
language = "English (US)",
journal = "ImmunoMethods",
issn = "1046-2023",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Intravital multiphoton microscopy as a tool for studying renal physiology and pathophysiology

AU - Sandoval, Ruben M.

AU - Molitoris, Bruce

PY - 2017

Y1 - 2017

N2 - The kidney is a complex and dynamic organ with over 40 cell types, and tremendous structural and functional diversity. Intravital multi-photon microscopy, development of fluorescent probes and innovative software, have rapidly advanced the study of intracellular and intercellular processes within the kidney. Researchers can quantify the distribution, behavior, and dynamic interactions of up to four labeled chemical probes and proteins simultaneously and repeatedly in four dimensions (time), with subcellular resolution in near real time. Thus, multi-photon microscopy has greatly extended our ability to investigate cell biology intravitally, at cellular and subcellular resolutions. Therefore, the purpose of the chapter is to demonstrate how the use in intravital multi-photon microscopy has advanced the understanding of both the physiology and pathophysiology of the kidney.

AB - The kidney is a complex and dynamic organ with over 40 cell types, and tremendous structural and functional diversity. Intravital multi-photon microscopy, development of fluorescent probes and innovative software, have rapidly advanced the study of intracellular and intercellular processes within the kidney. Researchers can quantify the distribution, behavior, and dynamic interactions of up to four labeled chemical probes and proteins simultaneously and repeatedly in four dimensions (time), with subcellular resolution in near real time. Thus, multi-photon microscopy has greatly extended our ability to investigate cell biology intravitally, at cellular and subcellular resolutions. Therefore, the purpose of the chapter is to demonstrate how the use in intravital multi-photon microscopy has advanced the understanding of both the physiology and pathophysiology of the kidney.

KW - Endocytosis

KW - Glomerular filtration

KW - Kidney

KW - Microvasculature

KW - Structure-function relationship

UR - http://www.scopus.com/inward/record.url?scp=85025132727&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85025132727&partnerID=8YFLogxK

U2 - 10.1016/j.ymeth.2017.07.014

DO - 10.1016/j.ymeth.2017.07.014

M3 - Article

C2 - 28733090

AN - SCOPUS:85025132727

JO - ImmunoMethods

JF - ImmunoMethods

SN - 1046-2023

ER -