Intravital multiphoton microscopy as a tool for studying renal physiology and pathophysiology

Ruben M. Sandoval, Bruce A. Molitoris

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


The kidney is a complex and dynamic organ with over 40 cell types, and tremendous structural and functional diversity. Intravital multi-photon microscopy, development of fluorescent probes and innovative software, have rapidly advanced the study of intracellular and intercellular processes within the kidney. Researchers can quantify the distribution, behavior, and dynamic interactions of up to four labeled chemical probes and proteins simultaneously and repeatedly in four dimensions (time), with subcellular resolution in near real time. Thus, multi-photon microscopy has greatly extended our ability to investigate cell biology intravitally, at cellular and subcellular resolutions. Therefore, the purpose of the chapter is to demonstrate how the use in intravital multi-photon microscopy has advanced the understanding of both the physiology and pathophysiology of the kidney.

Original languageEnglish (US)
Pages (from-to)20-32
Number of pages13
StatePublished - Sep 1 2017


  • Endocytosis
  • Glomerular filtration
  • Kidney
  • Microvasculature
  • Structure-function relationship

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Intravital multiphoton microscopy as a tool for studying renal physiology and pathophysiology'. Together they form a unique fingerprint.

Cite this