Intravital multiphoton microscopy of dynamic renal processes

Bruce Molitoris, Ruben M. Sandoval

Research output: Contribution to journalArticle

133 Citations (Scopus)

Abstract

Recent advances in microscopy and optics, computer sciences, and the available fluorophores used to label molecules of interest have empowered investigators to utilize intravital two-photon microscopy to study the dynamic events within the functioning kidney. This emerging technique enables investigators to follow functional and structural alterations with subcellular resolution within the same field of view over seconds to weeks. This approach invigorates the validity of data and facilitates analysis and interpretation as trends are more readily determined when one is more closely monitoring indicative physiological parameters. Therefore, in this review we emphasize how specific approaches will enable studies into glomerular permeability, proximal tubule endocytosis, and microvascular function within the kidney. We attempt to show how visual data can be quantified, thus allowing enhanced understanding of the process under study. Finally, emphasis is given to the possible future opportunities of this technology and its present limitations.

Original languageEnglish
JournalAmerican Journal of Physiology - Renal Physiology
Volume288
Issue number6 57-6
DOIs
StatePublished - Jun 2005

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Microscopy
Research Personnel
Kidney
Physiologic Monitoring
Endocytosis
Photons
Permeability
Technology
Intravital Microscopy

Keywords

  • Endocytosis
  • Glomerular filtration
  • Proximal tubule cells

ASJC Scopus subject areas

  • Physiology

Cite this

Intravital multiphoton microscopy of dynamic renal processes. / Molitoris, Bruce; Sandoval, Ruben M.

In: American Journal of Physiology - Renal Physiology, Vol. 288, No. 6 57-6, 06.2005.

Research output: Contribution to journalArticle

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