Intravitreal growth factors in proliferative diabetic retinopathy: Correlation with neovascular activity and glycaemic management

Mike Boulton, Zdenek Gregor, David McLeod, David Charteris, John Jarvis-Evans, Patrick Moriarty, Asud Khaliq, David Foreman, David Allamby, Bill Bardsley

Research output: Contribution to journalArticle

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Abstract

Aim - Many growth factors are implicated in proliferative diabetic retinopathy (PDR). It was decided to test the hypothesis that no one factor is predominant but that a regular profile of levels of different growth factors might be operating, and that the profile might differ according to whether or not insulin therapy was part of the patient's glycaemic management. The levels of several growth factors in vitrectomy samples were therefore determined from diabetic patients with tractional, non-haemorrhagic sequelae of PDR and these levels were correlated with (a) each other (growth factor profile), (b) neovascular activity, and (c) the method of glycaemic management (insulin treated (IT) or non-insulin treated (NIT)). Methods - 72 samples of vitreous were obtained from either diabetic patients with PDR (n = 51) or non-diabetic (control) patients (n = 21). Levels of bFGF, IGF-I, EGF, and insulin were determined by radioimmunoassay; levels of TGF-β2 by ELISA; and levels of IGF-I binding protein by western ligand blotting. The data were analysed using appropriate statistics. Results - There was no regular growth factor profile, bFGF levels were significantly greater in vitreous from NIT patients compared with IT patients and controls. The highest levels of bFGF were found in NIT patients with actively vascularised membranes. TGF-β2 levels were significantly greater in vitreous from IT patients compared with NIT patients and controls The highest levels of TGF-β2 were found in IT patients with actively vascularised membranes. IGF-I levels were significantly greater in diabetics (irrespective of insulin treatment) than non-diabetics and the highest levels of IGF-I were found in IT patients with actively vascularised membranes. A 34 kDa IGFBP was the predominant IGFBP identified in vitreous and was found to be elevated in diabetics patients. Conclusion - In PDR there is a correlation between intravitreal growth factor levels and both disease state (whether active or fibrotic) and method of glycaemic management.

Original languageEnglish (US)
Pages (from-to)228-233
Number of pages6
JournalBritish Journal of Ophthalmology
Volume81
Issue number3
StatePublished - 1997
Externally publishedYes

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Diabetic Retinopathy
Intercellular Signaling Peptides and Proteins
Insulin
Insulin-Like Growth Factor I
Insulin-Like Growth Factor Binding Proteins
Membranes
Vitrectomy
Epidermal Growth Factor
Radioimmunoassay
Western Blotting
Enzyme-Linked Immunosorbent Assay
Ligands

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Boulton, M., Gregor, Z., McLeod, D., Charteris, D., Jarvis-Evans, J., Moriarty, P., ... Bardsley, B. (1997). Intravitreal growth factors in proliferative diabetic retinopathy: Correlation with neovascular activity and glycaemic management. British Journal of Ophthalmology, 81(3), 228-233.

Intravitreal growth factors in proliferative diabetic retinopathy : Correlation with neovascular activity and glycaemic management. / Boulton, Mike; Gregor, Zdenek; McLeod, David; Charteris, David; Jarvis-Evans, John; Moriarty, Patrick; Khaliq, Asud; Foreman, David; Allamby, David; Bardsley, Bill.

In: British Journal of Ophthalmology, Vol. 81, No. 3, 1997, p. 228-233.

Research output: Contribution to journalArticle

Boulton, M, Gregor, Z, McLeod, D, Charteris, D, Jarvis-Evans, J, Moriarty, P, Khaliq, A, Foreman, D, Allamby, D & Bardsley, B 1997, 'Intravitreal growth factors in proliferative diabetic retinopathy: Correlation with neovascular activity and glycaemic management', British Journal of Ophthalmology, vol. 81, no. 3, pp. 228-233.
Boulton, Mike ; Gregor, Zdenek ; McLeod, David ; Charteris, David ; Jarvis-Evans, John ; Moriarty, Patrick ; Khaliq, Asud ; Foreman, David ; Allamby, David ; Bardsley, Bill. / Intravitreal growth factors in proliferative diabetic retinopathy : Correlation with neovascular activity and glycaemic management. In: British Journal of Ophthalmology. 1997 ; Vol. 81, No. 3. pp. 228-233.
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abstract = "Aim - Many growth factors are implicated in proliferative diabetic retinopathy (PDR). It was decided to test the hypothesis that no one factor is predominant but that a regular profile of levels of different growth factors might be operating, and that the profile might differ according to whether or not insulin therapy was part of the patient's glycaemic management. The levels of several growth factors in vitrectomy samples were therefore determined from diabetic patients with tractional, non-haemorrhagic sequelae of PDR and these levels were correlated with (a) each other (growth factor profile), (b) neovascular activity, and (c) the method of glycaemic management (insulin treated (IT) or non-insulin treated (NIT)). Methods - 72 samples of vitreous were obtained from either diabetic patients with PDR (n = 51) or non-diabetic (control) patients (n = 21). Levels of bFGF, IGF-I, EGF, and insulin were determined by radioimmunoassay; levels of TGF-β2 by ELISA; and levels of IGF-I binding protein by western ligand blotting. The data were analysed using appropriate statistics. Results - There was no regular growth factor profile, bFGF levels were significantly greater in vitreous from NIT patients compared with IT patients and controls. The highest levels of bFGF were found in NIT patients with actively vascularised membranes. TGF-β2 levels were significantly greater in vitreous from IT patients compared with NIT patients and controls The highest levels of TGF-β2 were found in IT patients with actively vascularised membranes. IGF-I levels were significantly greater in diabetics (irrespective of insulin treatment) than non-diabetics and the highest levels of IGF-I were found in IT patients with actively vascularised membranes. A 34 kDa IGFBP was the predominant IGFBP identified in vitreous and was found to be elevated in diabetics patients. Conclusion - In PDR there is a correlation between intravitreal growth factor levels and both disease state (whether active or fibrotic) and method of glycaemic management.",
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T1 - Intravitreal growth factors in proliferative diabetic retinopathy

T2 - Correlation with neovascular activity and glycaemic management

AU - Boulton, Mike

AU - Gregor, Zdenek

AU - McLeod, David

AU - Charteris, David

AU - Jarvis-Evans, John

AU - Moriarty, Patrick

AU - Khaliq, Asud

AU - Foreman, David

AU - Allamby, David

AU - Bardsley, Bill

PY - 1997

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