Introduction of large insert DNA into mammalian cells and embryos.

R. H. Reeves, D. E. Cabin, Bruce Lamb

Research output: Contribution to journalArticle

Abstract

This unit provides a set of protocols for introducing large insert DNA into cultured mammalian cells and embryos. Two different methods, spheroplast fusion and lipofection, are described for effecting transfer of YACs or gel-purified YAC DNA into cells. Additional protocols discuss preparing and transferring BACs into cells by lipofection and into embryos by microinjection.

Original languageEnglish (US)
JournalCurrent protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.]
VolumeChapter 5
StatePublished - Nov 2001
Externally publishedYes

Fingerprint

Mammalian Embryo
Spheroplasts
DNA
Microinjections
Cultured Cells
Embryonic Structures
Gels

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Introduction of large insert DNA into mammalian cells and embryos. / Reeves, R. H.; Cabin, D. E.; Lamb, Bruce.

In: Current protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.], Vol. Chapter 5, 11.2001.

Research output: Contribution to journalArticle

@article{7c681f1e9ffc416ca43d91e28235abc7,
title = "Introduction of large insert DNA into mammalian cells and embryos.",
abstract = "This unit provides a set of protocols for introducing large insert DNA into cultured mammalian cells and embryos. Two different methods, spheroplast fusion and lipofection, are described for effecting transfer of YACs or gel-purified YAC DNA into cells. Additional protocols discuss preparing and transferring BACs into cells by lipofection and into embryos by microinjection.",
author = "Reeves, {R. H.} and Cabin, {D. E.} and Bruce Lamb",
year = "2001",
month = "11",
language = "English (US)",
volume = "Chapter 5",
journal = "Current Protocols in Human Genetics",
issn = "1934-8266",
publisher = "John Wiley and Sons Inc.",

}

TY - JOUR

T1 - Introduction of large insert DNA into mammalian cells and embryos.

AU - Reeves, R. H.

AU - Cabin, D. E.

AU - Lamb, Bruce

PY - 2001/11

Y1 - 2001/11

N2 - This unit provides a set of protocols for introducing large insert DNA into cultured mammalian cells and embryos. Two different methods, spheroplast fusion and lipofection, are described for effecting transfer of YACs or gel-purified YAC DNA into cells. Additional protocols discuss preparing and transferring BACs into cells by lipofection and into embryos by microinjection.

AB - This unit provides a set of protocols for introducing large insert DNA into cultured mammalian cells and embryos. Two different methods, spheroplast fusion and lipofection, are described for effecting transfer of YACs or gel-purified YAC DNA into cells. Additional protocols discuss preparing and transferring BACs into cells by lipofection and into embryos by microinjection.

UR - http://www.scopus.com/inward/record.url?scp=44049102058&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=44049102058&partnerID=8YFLogxK

M3 - Article

VL - Chapter 5

JO - Current Protocols in Human Genetics

JF - Current Protocols in Human Genetics

SN - 1934-8266

ER -