Introduction of large insert DNA into mammalian cells and embryos.

R. H. Reeves, D. E. Cabin, B. Lamb

Research output: Contribution to journalArticle

Abstract

This unit provides a set of protocols for introducing large insert DNA into cultured mammalian cells and embryos. Two different methods, spheroplast fusion and lipofection, are described for effecting transfer of YACs or gel-purified YAC DNA into cells. Additional protocols discuss preparing and transferring BACs into cells by lipofection and into embryos by microinjection.

Original languageEnglish (US)
Pages (from-to)Unit 5.12
JournalCurrent protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.]
VolumeChapter 5
DOIs
StatePublished - Nov 2001
Externally publishedYes

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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