Invasive and angiogenic phenotype of MCF-7 human breast tumor cells expressing human cyclooxygenase-2

Jenifer Prosperi, Susan R. Mallery, Kristina A. Kigerl, Abigail A. Erfurt, Fredika M. Robertson

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

To evaluate the direct effect of human cyclooxygenase-2 (hCox-2) on human breast tumor cell proliferation, invasion, and angiogenesis, hCox-2 cDNA was transfected into slow growing, non-metastatic MCF-7 human breast tumor cells that express low levels of Cox-2. Two stable transfectant clones, designated MCF-7/hCox-2 clones 8 and 10, had significantly decreased (P145 and V165. These results demonstrate that hCox-2 directly increases breast tumor cell proliferation, stimulates invasion across a basement membrane, and induces synthesis of specific heparin binding splice variants of VEGF.

Original languageEnglish (US)
Pages (from-to)249-264
Number of pages16
JournalProstaglandins and Other Lipid Mediators
Volume73
Issue number3-4
DOIs
StatePublished - Apr 2004
Externally publishedYes

Fingerprint

Cyclooxygenase 2
Tumors
Cells
Breast Neoplasms
Phenotype
Cell proliferation
Clone Cells
Cell Proliferation
Vascular Endothelial Growth Factor A
Heparin
Complementary DNA
Basement Membrane

Keywords

  • hEGF
  • Human epidermal growth factor
  • Matrix metalloproteinase-2
  • Membrane type matrix metalloproteinase 1
  • MMLV-RT
  • MMP-2
  • Moloney murine leukemia virus reverse transcriptase
  • MT-MMP-1
  • PCR
  • Polymerase chain reaction
  • Vascular endothelial growth factor
  • VEGF

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

Invasive and angiogenic phenotype of MCF-7 human breast tumor cells expressing human cyclooxygenase-2. / Prosperi, Jenifer; Mallery, Susan R.; Kigerl, Kristina A.; Erfurt, Abigail A.; Robertson, Fredika M.

In: Prostaglandins and Other Lipid Mediators, Vol. 73, No. 3-4, 04.2004, p. 249-264.

Research output: Contribution to journalArticle

Prosperi, Jenifer ; Mallery, Susan R. ; Kigerl, Kristina A. ; Erfurt, Abigail A. ; Robertson, Fredika M. / Invasive and angiogenic phenotype of MCF-7 human breast tumor cells expressing human cyclooxygenase-2. In: Prostaglandins and Other Lipid Mediators. 2004 ; Vol. 73, No. 3-4. pp. 249-264.
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