Inversin forms a complex with catenins and N-cadherin in polarized epithelial cells

Jens Nürnberger, Robert Bacallao, Carrie Phillips

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

Nephrogenesis starts with the reciprocal induction of two embryonically distinct analages, metanephric mesenchyme and ureteric bud. This complex process requires the refined and coordinated expression of numerous developmental genes, such as inv. Mice that are homozygous for a mutation in the inv gene (inv/inv) develop renal cysts resembling autosomal-recessive polycystic kidney disease. The gene locus containing inv has been proposed to serve as a common modifier for some human and rodent polycystic kidney disease phenotypes. We generated polyclonal antibodies to inversin to study its subcellular distribution, potential binding partners, and functional aspects in cultured murine proximal tubule cells. A 125-kDa inversin protein isoform was found at cell-cell junctions. Two inversin isoforms, 140- and 90-kDa, were identified in the nuclear and perinuclear compartments. Plasma membrane allocation of inversin is dependent upon cell-cell contacts and was redistributed when cell adhesion was disrupted after incubation of the cell monolayer with low-calcium/EGTA medium. We further show that the membrane-associated 125-kDa inversin forms a complex with N-cadherin and the catenins. The 90-kDa nuclear inversin complexes with β-catenin. These findings indicate that the inv gene product functions in several cellular compartments, including the nucleus and cell-cell adhesion sites.

Original languageEnglish
Pages (from-to)3096-3106
Number of pages11
JournalMolecular Biology of the Cell
Volume13
Issue number9
DOIs
StatePublished - Sep 2002

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Catenins
Cadherins
Epithelial Cells
Cell Adhesion
Protein Isoforms
Autosomal Recessive Polycystic Kidney
Genes
Developmental Genes
Polycystic Kidney Diseases
Intercellular Junctions
Egtazic Acid
Mesoderm
Cell Nucleus
Cysts
Rodentia
Cell Membrane
Calcium
Phenotype
Kidney
Mutation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Inversin forms a complex with catenins and N-cadherin in polarized epithelial cells. / Nürnberger, Jens; Bacallao, Robert; Phillips, Carrie.

In: Molecular Biology of the Cell, Vol. 13, No. 9, 09.2002, p. 3096-3106.

Research output: Contribution to journalArticle

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