Inverted Alu dsRNA structures do not affect localization but can alter translation efficiency of human mRNAs independent of RNA editing

Claire R. Capshew, Kristen L. Dusenbury, Heather Hundley

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

With over one million copies, Alu elements are the most abundant repetitive elements in the human genome. When transcribed, interaction between two Alus that are in opposite orientation gives rise to double-stranded RNA (dsRNA). Although the presence of dsRNA in the cell was previously thought to only occur during viral infection, it is now known that cells express many endogenous small dsRNAs, such as short interfering RNA (siRNAs) and microRNA (miRNAs), which regulate gene expression. It is possible that long dsRNA structures formed from Alu elements influence gene expression. Here, we report that human mRNAs containing inverted Alu elements are present in the mammalian cytoplasm. The presence of these long intramolecular dsRNA structures within 3′-UTRs decreases translational efficiency, and although the structures undergo extensive editing in vivo, the effects on translation are independent of the presence of inosine. As inverted Alus are predicted to reside in >5 of human protein-coding genes, these intramolecular dsRNA structures are important regulators of gene expression.

Original languageEnglish
Pages (from-to)8637-8645
Number of pages9
JournalNucleic Acids Research
Volume40
Issue number17
DOIs
StatePublished - Sep 2012

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RNA Editing
Double-Stranded RNA
Alu Elements
Messenger RNA
Gene Expression
Inosine
3' Untranslated Regions
Human Genome
Virus Diseases
Regulator Genes
MicroRNAs
Small Interfering RNA
Cytoplasm
Proteins

ASJC Scopus subject areas

  • Genetics

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Inverted Alu dsRNA structures do not affect localization but can alter translation efficiency of human mRNAs independent of RNA editing. / Capshew, Claire R.; Dusenbury, Kristen L.; Hundley, Heather.

In: Nucleic Acids Research, Vol. 40, No. 17, 09.2012, p. 8637-8645.

Research output: Contribution to journalArticle

Capshew, Claire R. ; Dusenbury, Kristen L. ; Hundley, Heather. / Inverted Alu dsRNA structures do not affect localization but can alter translation efficiency of human mRNAs independent of RNA editing. In: Nucleic Acids Research. 2012 ; Vol. 40, No. 17. pp. 8637-8645.
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