Involvement of glutamate in transmission of afferent constrictive inputs from the airways to the nucleus tractus solitarius in ferrets

Musa A. Haxhiu, Bryan Yamamoto, Ismail A. Dreshaj, David Bedol, Donald G. Ferguson

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

In this study, we identified the neurons within nucleus tractus solitarius (nTS) activated by stimulation of airway sensory systems and examined the expression of AMPA receptor subtype(s) by these cells. We also investigated the possible involvement of endogenously released glutamate and AMPA receptors in the transmission of excitatory inputs from the sensory system of the respiratory tract to the neurons of the nTS. In these experiments we used: (1) immunodetection of c-fos encoded protein (cFos) expression to identify the nTS neurons activated by the stimulation of the airway sensory system; (2) receptor immunochemistry and confocal microscopy to determine the receptor(s) expressed by activated nTS neurons; (3) microdialysis to measure glutamate release, and (4) physiological measurements to examine the effects of selective receptor blockers, and thereby define the role of the glutamate and AMPA glutamatergic receptor subtype(s) in reflexly induced airway constriction. The results showed that activation of airway sensory receptors, by inhalation of aerosolized histamine or capsaicin, induced cFos expression in a subset of nTS neurons that also expressed the AMPA subtype of glutamate receptors. Furthermore, activation of sensory bronchoconstrictive receptors induced glutamate release within nTS, and blockade of the AMPA receptor subtype within nTS inhibited reflexly increased cholinergic outflow to the airways. These data indicate for the first time that glutamate and AMPA receptor signaling pathways are involved in the transmission of afferent inputs from the airways to the nTS, and in mediating reflex airway constriction. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)22-30
Number of pages9
JournalJournal of the Autonomic Nervous System
Volume80
Issue number1-2
DOIs
StatePublished - Apr 12 2000
Externally publishedYes

Fingerprint

Ferrets
Solitary Nucleus
Glutamic Acid
AMPA Receptors
Glutamate Receptors
Neurons
Sensory Receptor Cells
Constriction
Proto-Oncogene Proteins c-fos
Immunochemistry
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Capsaicin
Microdialysis
Confocal Microscopy
Respiratory System
Cholinergic Agents
Histamine
Inhalation
Reflex

Keywords

  • Airway reflexes
  • AMPA receptors
  • c-fos
  • L-glutamate
  • Microdialysis
  • Nucleus tractus solitarius, nTS
  • Parasympathetic tone

ASJC Scopus subject areas

  • Physiology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Involvement of glutamate in transmission of afferent constrictive inputs from the airways to the nucleus tractus solitarius in ferrets. / Haxhiu, Musa A.; Yamamoto, Bryan; Dreshaj, Ismail A.; Bedol, David; Ferguson, Donald G.

In: Journal of the Autonomic Nervous System, Vol. 80, No. 1-2, 12.04.2000, p. 22-30.

Research output: Contribution to journalArticle

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