Involvement of the nitric oxide-cyclic GMP pathway in the desensitization of bradykinin responses of cultured rat sensory neurons

Daniel S. McGehee, Michael F. Goy, Gerry S. Oxford

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Bradykinin (BK) excites a subset of dorsal root ganglion neurons by inducing an inward cation current (IBK) that strongly desensitizes and is accompanied by elevations in cGMP. We have examined the links between cGMP metabolism and IBK. The BK dose dependencies of IBK activation, desensitization, and cGMP production are comparable. Stimulation (with sodium nitroprusside [NP] or 8-bromo-cGMP [88r-cGMP]) or inhibition (with methylene blue, hemoglobin, and nitric oxide synthase [NOS] inhibitors) of cGMP levels did not mimic or diminish IBK. However, desensitization was affected by the following agents: first, desensitization was enhanced by NP and reduced by NOS inhibitors. Second, the effects of NOS inhibitors could be overcome by 8Br-cGMP or l-arginine. Third, 8Br-cGMP modification of desensitization required receptor occupancy. We conclude that the NO-cGMP pathway affects a component of IBK desensitization at the receptor or G protein level.

Original languageEnglish (US)
Pages (from-to)315-324
Number of pages10
JournalNeuron
Volume9
Issue number2
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

Cyclic GMP
Bradykinin
Sensory Receptor Cells
Nitric Oxide Synthase
Nitric Oxide
Nitroprusside
Methylene Blue
Spinal Ganglia
GTP-Binding Proteins
Arginine
Cations
Hemoglobins
Neurons

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Involvement of the nitric oxide-cyclic GMP pathway in the desensitization of bradykinin responses of cultured rat sensory neurons. / McGehee, Daniel S.; Goy, Michael F.; Oxford, Gerry S.

In: Neuron, Vol. 9, No. 2, 1992, p. 315-324.

Research output: Contribution to journalArticle

McGehee, Daniel S. ; Goy, Michael F. ; Oxford, Gerry S. / Involvement of the nitric oxide-cyclic GMP pathway in the desensitization of bradykinin responses of cultured rat sensory neurons. In: Neuron. 1992 ; Vol. 9, No. 2. pp. 315-324.
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