Ionophore-resistant mutants of Toxoplasma gondii reveal host cell permeabilization as an early event in egress

M. W. Black, Gustavo Arrizabalaga, J. C. Boothroyd

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Abstract

Toxoplasma gondii is an obligate intracellular pathogen within the phylum Apicomplexa. Invasion and egress by this protozoan parasite are rapid events that are dependent upon parasite motility and appear to be directed by fluctuations in intracellular [Ca2+]. Treatment of infected host cells with the calcium ionophore A23187 causes the parasites to undergo rapid egress in a process termed ionophore-induced egress (IIE). In contrast, when extracellular parasites are exposed to this ionophore, they quickly lose infectivity (termed ionophore-induced death [IID]). From among several Iie- mutants described here, two were identified that differ in several attributes, most notably in their resistance to IID. The association between the Iie- and Iid- phenotypes is supported by the observation that two-thirds of mutants selected as Iid- are also Iie-. Characterization of three distinct classes of IIE and IID mutants revealed that the Iie- phenotype is due to a defect in a parasite-dependent activity that normally causes infected host cells to be permeabilized just prior to egress. Iie- parasites underwent rapid egress when infected cells were artificially permeabilized by a mild saponin treatment, confirming that this step is deficient in the Iie- mutants. A model is proposed that includes host cell permeabilization as a critical part of the signaling pathway leading to parasite egress. The fact that Iie- mutants are also defective in early stages of the lyric cycle indicates some commonality between these normal processes and IIE.

Original languageEnglish (US)
Pages (from-to)9399-9408
Number of pages10
JournalMolecular and Cellular Biology
Volume20
Issue number24
DOIs
StatePublished - 2000
Externally publishedYes

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Ionophores
Toxoplasma
Parasites
Apicomplexa
Phenotype
Calcium Ionophores
Saponins
Calcimycin

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Ionophore-resistant mutants of Toxoplasma gondii reveal host cell permeabilization as an early event in egress. / Black, M. W.; Arrizabalaga, Gustavo; Boothroyd, J. C.

In: Molecular and Cellular Biology, Vol. 20, No. 24, 2000, p. 9399-9408.

Research output: Contribution to journalArticle

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