Iron chelation acutely stimulates fetal human intestinal cell production of IL-6 and VEGF while decreasing HGF: The roles of p38, ERK, and JNK MAPK signaling

Troy A. Markel, Paul R. Crisostomo, Meijing Wang, Christine M. Herring, Tim Lahm, Kirstan K. Meldrum, Keith D. Lillemoe, Frederick Rescorla, Daniel R. Meldrum

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Bacteria have developed mechanisms to sequester host iron via chelators such as deferoxamine (DFO). Interestingly, DFO has been shown to stimulate acute intestinal epithelial cell inflammatory cytokine production in the absence of bacteria; however, this mechanism has not been elucidated. Intestinal epithelial cell production of IL-6 and TNF-α is elevated in various gastrointestinal pathologies, including acute intestinal ischemia. Similarly, VEGF and HGF are essential to intestinal epithelial cell integrity. Therapeutic strategies that decrease IL-6 and TNF-α while increasing VEGF and HGF therefore have theoretical appeal. We hypothesized that 1) fetal human intestinal epithelial cells acutely produce increased IL-6, TNF-α, VEGF, and HGF during iron chelation and 2) the MAPK pathway mediates these effects. Fetal human intestinal epithelial cells were stimulated by iron chelation (1 mM DFO) with and without p38 MAPK, ERK, or JNK inhibition. Supernatants were harvested after 24 h of incubation, and IL-6, TNF-α, VEGF, and HGF levels were quantified by ELISA. Activation of MAPK pathways was confirmed by Western blot analysis. DFO stimulation resulted in a significant increase in epithelial cell IL-6 and VEGF production while yielding a decrease in HGF production (P < 0.05). Unexpectedly, TNF-α was not detectable. p38 MAPK, ERK, and JNK inhibition significantly decreased IL-6, VEGF, and HGF production (P < 0.05). In conclusion, DFO acutely increases fetal human intestinal epithelial cell IL-6 and VEGF expression while causing an unexpected decrease in HGF expression and no detectable TNF-α production. Furthermore, chelator-induced intestinal epithelial cell cytokine expression depends on p38, ERK, and JNK MAPK pathways.

Original languageEnglish
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume292
Issue number4
DOIs
StatePublished - Apr 2007

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Vascular Endothelial Growth Factor A
Interleukin-6
Iron
Epithelial Cells
Deferoxamine
p38 Mitogen-Activated Protein Kinases
Chelating Agents
Cytokines
Bacteria
MAP Kinase Signaling System
Ischemia
Western Blotting
Enzyme-Linked Immunosorbent Assay
Pathology

Keywords

  • c-Jun NH-terminal kinase
  • Extracellular signal-regulated kinase
  • Hepatocyte growth factor
  • Inflammation
  • Interleukin-6
  • Intestinal ischemia
  • Mitogen-activated protein kinase signaling
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Gastroenterology
  • Physiology

Cite this

Iron chelation acutely stimulates fetal human intestinal cell production of IL-6 and VEGF while decreasing HGF : The roles of p38, ERK, and JNK MAPK signaling. / Markel, Troy A.; Crisostomo, Paul R.; Wang, Meijing; Herring, Christine M.; Lahm, Tim; Meldrum, Kirstan K.; Lillemoe, Keith D.; Rescorla, Frederick; Meldrum, Daniel R.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 292, No. 4, 04.2007.

Research output: Contribution to journalArticle

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abstract = "Bacteria have developed mechanisms to sequester host iron via chelators such as deferoxamine (DFO). Interestingly, DFO has been shown to stimulate acute intestinal epithelial cell inflammatory cytokine production in the absence of bacteria; however, this mechanism has not been elucidated. Intestinal epithelial cell production of IL-6 and TNF-α is elevated in various gastrointestinal pathologies, including acute intestinal ischemia. Similarly, VEGF and HGF are essential to intestinal epithelial cell integrity. Therapeutic strategies that decrease IL-6 and TNF-α while increasing VEGF and HGF therefore have theoretical appeal. We hypothesized that 1) fetal human intestinal epithelial cells acutely produce increased IL-6, TNF-α, VEGF, and HGF during iron chelation and 2) the MAPK pathway mediates these effects. Fetal human intestinal epithelial cells were stimulated by iron chelation (1 mM DFO) with and without p38 MAPK, ERK, or JNK inhibition. Supernatants were harvested after 24 h of incubation, and IL-6, TNF-α, VEGF, and HGF levels were quantified by ELISA. Activation of MAPK pathways was confirmed by Western blot analysis. DFO stimulation resulted in a significant increase in epithelial cell IL-6 and VEGF production while yielding a decrease in HGF production (P < 0.05). Unexpectedly, TNF-α was not detectable. p38 MAPK, ERK, and JNK inhibition significantly decreased IL-6, VEGF, and HGF production (P < 0.05). In conclusion, DFO acutely increases fetal human intestinal epithelial cell IL-6 and VEGF expression while causing an unexpected decrease in HGF expression and no detectable TNF-α production. Furthermore, chelator-induced intestinal epithelial cell cytokine expression depends on p38, ERK, and JNK MAPK pathways.",
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T2 - The roles of p38, ERK, and JNK MAPK signaling

AU - Markel, Troy A.

AU - Crisostomo, Paul R.

AU - Wang, Meijing

AU - Herring, Christine M.

AU - Lahm, Tim

AU - Meldrum, Kirstan K.

AU - Lillemoe, Keith D.

AU - Rescorla, Frederick

AU - Meldrum, Daniel R.

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AB - Bacteria have developed mechanisms to sequester host iron via chelators such as deferoxamine (DFO). Interestingly, DFO has been shown to stimulate acute intestinal epithelial cell inflammatory cytokine production in the absence of bacteria; however, this mechanism has not been elucidated. Intestinal epithelial cell production of IL-6 and TNF-α is elevated in various gastrointestinal pathologies, including acute intestinal ischemia. Similarly, VEGF and HGF are essential to intestinal epithelial cell integrity. Therapeutic strategies that decrease IL-6 and TNF-α while increasing VEGF and HGF therefore have theoretical appeal. We hypothesized that 1) fetal human intestinal epithelial cells acutely produce increased IL-6, TNF-α, VEGF, and HGF during iron chelation and 2) the MAPK pathway mediates these effects. Fetal human intestinal epithelial cells were stimulated by iron chelation (1 mM DFO) with and without p38 MAPK, ERK, or JNK inhibition. Supernatants were harvested after 24 h of incubation, and IL-6, TNF-α, VEGF, and HGF levels were quantified by ELISA. Activation of MAPK pathways was confirmed by Western blot analysis. DFO stimulation resulted in a significant increase in epithelial cell IL-6 and VEGF production while yielding a decrease in HGF production (P < 0.05). Unexpectedly, TNF-α was not detectable. p38 MAPK, ERK, and JNK inhibition significantly decreased IL-6, VEGF, and HGF production (P < 0.05). In conclusion, DFO acutely increases fetal human intestinal epithelial cell IL-6 and VEGF expression while causing an unexpected decrease in HGF expression and no detectable TNF-α production. Furthermore, chelator-induced intestinal epithelial cell cytokine expression depends on p38, ERK, and JNK MAPK pathways.

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KW - Vascular endothelial growth factor

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