Iron deficiency suppresses ileal nitric oxide synthase activity.

M. I. Goldblatt, S. H. Choi, D. A. Swartz-Basile, Attila Nakeeb, S. K. Sarna, H. A. Pitt

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Intestinal motility disorders are more common in women of childbearing age who are prone to iron deficiency anemia. The neurotransmitters nitric oxide (NO) and acetylcholine (ACh) play a key role in ileal smooth muscle relaxation and contraction, respectively. Iron-containing heme is known to be a cofactor for nitric oxide synthase (NOS), the enzyme responsible for NO production. Therefore we tested the hypothesis that iron deficiency would downregulate ileal NOS activity without affecting the ileum's response to ACh. Twelve adult female prairie dogs were fed either an iron-supplemented (Fe+) (200 ppm) (n = 6) or an iron-deficient (Fe-) (8 ppm) (n = 6) diet for 8 weeks. Ileal circular muscle strips were harvested to measure responses to ACh and electrical field stimulation. Under nonadrenergic noncholinergic (NANC) conditions, Nomega-nitro-L-arginine (L-NNA), an NOS inhibitor, and VIP(10-28), a vasoactive intestinal peptide (VIP) inhibitor, were added prior to electrical field stimulation. NANC inhibitory responses are expressed as a percentage of optimal relaxation from EDTA. The excitatory response to ACh was similar in both groups (1.1 +/- 0.3 N/cm(2) vs. 1.5 +/- 0.3 N/cm(2), P = 0.45). The inhibitory response to electrical field stimulation under NANC conditions was greater in the Fe+ group (34.7 +/- 2.9%) compared to the Fe- group (23.9 +/- 3.2%; P

Original languageEnglish (US)
JournalJournal of Gastrointestinal Surgery
Volume5
Issue number4
StatePublished - Jul 2001
Externally publishedYes

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Nitric Oxide Synthase
Acetylcholine
Iron
Electric Stimulation
Nitric Oxide
Sciuridae
Gastrointestinal Motility
Muscle Relaxation
Iron-Deficiency Anemias
Vasoactive Intestinal Peptide
Muscle Contraction
Heme
Ileum
Edetic Acid
Smooth Muscle
Neurotransmitter Agents
Arginine
Down-Regulation
Diet
Muscles

ASJC Scopus subject areas

  • Surgery

Cite this

Goldblatt, M. I., Choi, S. H., Swartz-Basile, D. A., Nakeeb, A., Sarna, S. K., & Pitt, H. A. (2001). Iron deficiency suppresses ileal nitric oxide synthase activity. Journal of Gastrointestinal Surgery, 5(4).

Iron deficiency suppresses ileal nitric oxide synthase activity. / Goldblatt, M. I.; Choi, S. H.; Swartz-Basile, D. A.; Nakeeb, Attila; Sarna, S. K.; Pitt, H. A.

In: Journal of Gastrointestinal Surgery, Vol. 5, No. 4, 07.2001.

Research output: Contribution to journalArticle

Goldblatt, MI, Choi, SH, Swartz-Basile, DA, Nakeeb, A, Sarna, SK & Pitt, HA 2001, 'Iron deficiency suppresses ileal nitric oxide synthase activity.', Journal of Gastrointestinal Surgery, vol. 5, no. 4.
Goldblatt MI, Choi SH, Swartz-Basile DA, Nakeeb A, Sarna SK, Pitt HA. Iron deficiency suppresses ileal nitric oxide synthase activity. Journal of Gastrointestinal Surgery. 2001 Jul;5(4).
Goldblatt, M. I. ; Choi, S. H. ; Swartz-Basile, D. A. ; Nakeeb, Attila ; Sarna, S. K. ; Pitt, H. A. / Iron deficiency suppresses ileal nitric oxide synthase activity. In: Journal of Gastrointestinal Surgery. 2001 ; Vol. 5, No. 4.
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