Is there a significant somatodendritic uptake of dopamine in the substantia nigra? Evidence from the weaver mutant mouse

J. R. Simon, Bernardino Ghetti

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Slices of the substantia nigra from normal mice and from mice with the weaver gene were used to study somatodendritic uptake of dopamine. The substantia nigra of the homozygous weaver mouse is deficient in both cell bodies and dendrites whereas the substantia nigra of the heterozygous weaver mouse is deficient only in dendrites. Accumulation of [3H]dopamine by nigral slices obtained from each genotype was not different from that observed in slices obtained from control mice. The observed accumulation of [3H]dopamine was apparently taking place predominantly into serotonergic and noradrenergic elements since significant reductions were obtained by both fluoxetine and desipramine at concentrations that were selective for the serotonin and norepinephrine carriers, respectively. In the absence, as well as in the presence of fluoxetine and desipramine, dopamine accumulation in the substantia nigra was only slightly attenuated by the known dopamine uptake blocker GBR 12909. These data argue against a significant presence of somatodendritic uptake systems for dopamine in the substantia nigra and suggest that caution be used in the interpretation of results from studies on dopamine release from nigral slices when [3H]dopamine has been used to preload the tissue. Under such experimental conditions, it is likely that a large proportion of the released tritium might come from neurons other than those which contain endogenous neurotransmitter dopamine.

Original languageEnglish
Pages (from-to)471-477
Number of pages7
JournalNeurochemistry International
Volume22
Issue number5
DOIs
StatePublished - 1993

Fingerprint

Neurologic Mutant Mice
Substantia Nigra
Dopamine
Desipramine
Fluoxetine
Dendrites
Dopamine Antagonists
Tritium
Neurotransmitter Agents
Serotonin
Norepinephrine
Genotype
Neurons

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

@article{073c4d2e0fd84194a9d8d1d7119caed0,
title = "Is there a significant somatodendritic uptake of dopamine in the substantia nigra? Evidence from the weaver mutant mouse",
abstract = "Slices of the substantia nigra from normal mice and from mice with the weaver gene were used to study somatodendritic uptake of dopamine. The substantia nigra of the homozygous weaver mouse is deficient in both cell bodies and dendrites whereas the substantia nigra of the heterozygous weaver mouse is deficient only in dendrites. Accumulation of [3H]dopamine by nigral slices obtained from each genotype was not different from that observed in slices obtained from control mice. The observed accumulation of [3H]dopamine was apparently taking place predominantly into serotonergic and noradrenergic elements since significant reductions were obtained by both fluoxetine and desipramine at concentrations that were selective for the serotonin and norepinephrine carriers, respectively. In the absence, as well as in the presence of fluoxetine and desipramine, dopamine accumulation in the substantia nigra was only slightly attenuated by the known dopamine uptake blocker GBR 12909. These data argue against a significant presence of somatodendritic uptake systems for dopamine in the substantia nigra and suggest that caution be used in the interpretation of results from studies on dopamine release from nigral slices when [3H]dopamine has been used to preload the tissue. Under such experimental conditions, it is likely that a large proportion of the released tritium might come from neurons other than those which contain endogenous neurotransmitter dopamine.",
author = "Simon, {J. R.} and Bernardino Ghetti",
year = "1993",
doi = "10.1016/0197-0186(93)90042-4",
language = "English",
volume = "22",
pages = "471--477",
journal = "Neurochemistry International",
issn = "0197-0186",
publisher = "Elsevier Limited",
number = "5",

}

TY - JOUR

T1 - Is there a significant somatodendritic uptake of dopamine in the substantia nigra? Evidence from the weaver mutant mouse

AU - Simon, J. R.

AU - Ghetti, Bernardino

PY - 1993

Y1 - 1993

N2 - Slices of the substantia nigra from normal mice and from mice with the weaver gene were used to study somatodendritic uptake of dopamine. The substantia nigra of the homozygous weaver mouse is deficient in both cell bodies and dendrites whereas the substantia nigra of the heterozygous weaver mouse is deficient only in dendrites. Accumulation of [3H]dopamine by nigral slices obtained from each genotype was not different from that observed in slices obtained from control mice. The observed accumulation of [3H]dopamine was apparently taking place predominantly into serotonergic and noradrenergic elements since significant reductions were obtained by both fluoxetine and desipramine at concentrations that were selective for the serotonin and norepinephrine carriers, respectively. In the absence, as well as in the presence of fluoxetine and desipramine, dopamine accumulation in the substantia nigra was only slightly attenuated by the known dopamine uptake blocker GBR 12909. These data argue against a significant presence of somatodendritic uptake systems for dopamine in the substantia nigra and suggest that caution be used in the interpretation of results from studies on dopamine release from nigral slices when [3H]dopamine has been used to preload the tissue. Under such experimental conditions, it is likely that a large proportion of the released tritium might come from neurons other than those which contain endogenous neurotransmitter dopamine.

AB - Slices of the substantia nigra from normal mice and from mice with the weaver gene were used to study somatodendritic uptake of dopamine. The substantia nigra of the homozygous weaver mouse is deficient in both cell bodies and dendrites whereas the substantia nigra of the heterozygous weaver mouse is deficient only in dendrites. Accumulation of [3H]dopamine by nigral slices obtained from each genotype was not different from that observed in slices obtained from control mice. The observed accumulation of [3H]dopamine was apparently taking place predominantly into serotonergic and noradrenergic elements since significant reductions were obtained by both fluoxetine and desipramine at concentrations that were selective for the serotonin and norepinephrine carriers, respectively. In the absence, as well as in the presence of fluoxetine and desipramine, dopamine accumulation in the substantia nigra was only slightly attenuated by the known dopamine uptake blocker GBR 12909. These data argue against a significant presence of somatodendritic uptake systems for dopamine in the substantia nigra and suggest that caution be used in the interpretation of results from studies on dopamine release from nigral slices when [3H]dopamine has been used to preload the tissue. Under such experimental conditions, it is likely that a large proportion of the released tritium might come from neurons other than those which contain endogenous neurotransmitter dopamine.

UR - http://www.scopus.com/inward/record.url?scp=0027160069&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027160069&partnerID=8YFLogxK

U2 - 10.1016/0197-0186(93)90042-4

DO - 10.1016/0197-0186(93)90042-4

M3 - Article

C2 - 8485453

AN - SCOPUS:0027160069

VL - 22

SP - 471

EP - 477

JO - Neurochemistry International

JF - Neurochemistry International

SN - 0197-0186

IS - 5

ER -