Ischemic vascular damage can be repaired by healthy, but not diabetic, endothelial progenitor cells

Sergio Caballero, Nilanjana Sengupta, Aqeela Afzal, Kyung Hee Chang, Sergio Li Calzi, Dennis L. Guberski, Timothy S. Kern, Maria B. Grant

Research output: Contribution to journalArticle

174 Citations (Scopus)

Abstract

Endothelial precursor cells (EPCs) play a key role in vascular repair and maintenance, and their function is impeded in diabetes. We previously demonstrated that EPCs isolated from diabetic patients have a profound inability to migrate in vitro. We asked whether EPCs from normal individuals are better able to repopulate degenerate (acellular) retinal capillaries in chronic (diabetes) and acute (ischemia/reperfusion [I/R] injury and neonatal oxygen-induced retinopathy [OIR]) animal models of ocular vascular damage. Streptozotocin-induced diabetic mice, spontaneously diabetic BBZDR/Wor rats, adult mice with I/R injury, or neonatal mice with OIR were injected within the vitreous or the systemic circulation with fluorescently labeled CD34+ cells from either diabetic patients or ageand sex-matched healthy control subjects. At specific times after administering the cells, the degree of vascular repair of the acellular capillaries was evaluated immunohistologically and quantitated. In all four models, healthy human (hu)CD34+ cells attached and assimilated into vasculature, whereas cells from diabetic donors uniformly were unable to integrate into damaged vasculature. These studies demonstrate that healthy huCD34+ cells can effectively repair injured retina and that there is defective repair of vasculature in patients with diabetes. Defective EPCs may be amenable to pharmacological manipulation and restoration of the cells' natural robust reparative function.

Original languageEnglish (US)
Pages (from-to)960-967
Number of pages8
JournalDiabetes
Volume56
Issue number4
DOIs
StatePublished - Apr 2007
Externally publishedYes

Fingerprint

Blood Vessels
Endothelial Cells
Reperfusion Injury
Oxygen
Streptozocin
Retina
Endothelial Progenitor Cells
Healthy Volunteers
Animal Models
Maintenance
Tissue Donors
Pharmacology

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Caballero, S., Sengupta, N., Afzal, A., Chang, K. H., Li Calzi, S., Guberski, D. L., ... Grant, M. B. (2007). Ischemic vascular damage can be repaired by healthy, but not diabetic, endothelial progenitor cells. Diabetes, 56(4), 960-967. https://doi.org/10.2337/db06-1254

Ischemic vascular damage can be repaired by healthy, but not diabetic, endothelial progenitor cells. / Caballero, Sergio; Sengupta, Nilanjana; Afzal, Aqeela; Chang, Kyung Hee; Li Calzi, Sergio; Guberski, Dennis L.; Kern, Timothy S.; Grant, Maria B.

In: Diabetes, Vol. 56, No. 4, 04.2007, p. 960-967.

Research output: Contribution to journalArticle

Caballero, S, Sengupta, N, Afzal, A, Chang, KH, Li Calzi, S, Guberski, DL, Kern, TS & Grant, MB 2007, 'Ischemic vascular damage can be repaired by healthy, but not diabetic, endothelial progenitor cells', Diabetes, vol. 56, no. 4, pp. 960-967. https://doi.org/10.2337/db06-1254
Caballero S, Sengupta N, Afzal A, Chang KH, Li Calzi S, Guberski DL et al. Ischemic vascular damage can be repaired by healthy, but not diabetic, endothelial progenitor cells. Diabetes. 2007 Apr;56(4):960-967. https://doi.org/10.2337/db06-1254
Caballero, Sergio ; Sengupta, Nilanjana ; Afzal, Aqeela ; Chang, Kyung Hee ; Li Calzi, Sergio ; Guberski, Dennis L. ; Kern, Timothy S. ; Grant, Maria B. / Ischemic vascular damage can be repaired by healthy, but not diabetic, endothelial progenitor cells. In: Diabetes. 2007 ; Vol. 56, No. 4. pp. 960-967.
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