ISGF3, the transcriptional activator induced by interferon α, consists of multiple interacting polypeptide chains

X. Y. Fu, D. S. Kessler, S. A. Veals, D. E. Levy, J. E. Darnell

Research output: Contribution to journalArticle

295 Scopus citations


Interferon-stimulated gene factor 3 (ISGF3) is the ligand-dependent transcriptional activator that, in response to interferon treatment, is assembled in the cell cytoplasm, is translocated to the nucleus, and binds the consensus DNA site, the interferon-stimulated response element. We have purified ISGF3 and identified its constituent proteins: a DNA-binding protein of 48 kDa and three larger polypeptides (84, 91, and 113 kDa), which themselves do not have DNA-binding activity. The multisubunit structure of ISGF3 most likely reflects its participation in receiving a ligand-dependent signal, translocating to the nucleus, and binding to DNA to activate transcription.

Original languageEnglish (US)
Pages (from-to)8555-8559
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number21
StatePublished - Jan 1 1990



  • factor purification
  • interferon-dependent transcription factor
  • interferon-stimulated response element

ASJC Scopus subject areas

  • General

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