ISGF3, the transcriptional activator induced by interferon a, consists of multiple interacting polypeptide chains

Xin Yuan Fu, Daniel S. Kessler, Susan A. Veals, David E. Levy, J. E. Darnell

Research output: Contribution to journalArticle

290 Citations (Scopus)

Abstract

Interferon-stimulated gene factor 3 (ISGF3) is the ligand-dependent transcriptional activator that, in response to interferon treatment, is assembled in the cell cytoplasm, is translocated to the nucleus, and binds the consensus DNA site, the interferon-stimulated response element. We have purified ISGF3 and identified its constituent proteins: a DNA-binding protein of 48 kDa and three larger polypeptides (84, 91, and 113 kDa), which themselves do not have DNA-binding activity. The multisubunit structure of ISGF3 most likely reflects its participation in receiving a ligand-dependent signal, translocating to the nucleus, and binding to DNA to activate transcription.

Original languageEnglish (US)
Pages (from-to)8555-8559
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number21
StatePublished - 1990
Externally publishedYes

Fingerprint

Interferon-Stimulated Gene Factor 3
Interferons
Peptides
DNA
Ligands
DNA-Binding Proteins
Response Elements
Cytoplasm
Proteins

Keywords

  • Factor purification
  • Interferon-dependent transcription factor
  • Interferon-stimulatcd response element

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

ISGF3, the transcriptional activator induced by interferon a, consists of multiple interacting polypeptide chains. / Fu, Xin Yuan; Kessler, Daniel S.; Veals, Susan A.; Levy, David E.; Darnell, J. E.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, No. 21, 1990, p. 8555-8559.

Research output: Contribution to journalArticle

Fu, Xin Yuan ; Kessler, Daniel S. ; Veals, Susan A. ; Levy, David E. ; Darnell, J. E. / ISGF3, the transcriptional activator induced by interferon a, consists of multiple interacting polypeptide chains. In: Proceedings of the National Academy of Sciences of the United States of America. 1990 ; Vol. 87, No. 21. pp. 8555-8559.
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AU - Darnell, J. E.

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